TY - JOUR
T1 - Optimising Magnetic Sentinel Lymph Node Biopsy in an in vivo Porcine Model
AU - Ahmed, Muneer
AU - Anninga, Bauke
AU - Pouw, Joost J
AU - Vreeman, Suzan
AU - Peek, Mirjam
AU - Van Hemelrijck, Mieke
AU - Ten Haken, Bennie
AU - Pankhurst, Quentin
AU - Douek, Michael
PY - 2015/5
Y1 - 2015/5
N2 - The magnetic technique for sentinel lymph node biopsy (SLNB) has been evaluated in several clinical trials. An in vivo porcine model was developed to optimise the magnetic technique by evaluating the effect of differing volume, concentration and time of injection of magnetic tracer. A total of 60 sentinel node procedures were undertaken. There was a significant correlation between magnetometer counts and iron content of excised sentinel lymph nodes (SLNs) (r=0.82; P<0.001). Total number of SLNs increased with increasing volumes of magnetic tracer (P<0.001). Transcutaneous magnetometer counts increased with increasing time from injection of magnetic tracer (P<0.0001), plateauing within 60minutes. Increasing concentration resulted in higher iron content of SLNs (P=0.006), whilst a non-statistically significant trend was observed between volume injected and iron content of SLNs (P=0.07). Increasing magnetic tracer volume and injecting prior to surgery improves transcutaneous 'hotspot' identification but very high volumes, increase the number of nodes excised.
AB - The magnetic technique for sentinel lymph node biopsy (SLNB) has been evaluated in several clinical trials. An in vivo porcine model was developed to optimise the magnetic technique by evaluating the effect of differing volume, concentration and time of injection of magnetic tracer. A total of 60 sentinel node procedures were undertaken. There was a significant correlation between magnetometer counts and iron content of excised sentinel lymph nodes (SLNs) (r=0.82; P<0.001). Total number of SLNs increased with increasing volumes of magnetic tracer (P<0.001). Transcutaneous magnetometer counts increased with increasing time from injection of magnetic tracer (P<0.0001), plateauing within 60minutes. Increasing concentration resulted in higher iron content of SLNs (P=0.006), whilst a non-statistically significant trend was observed between volume injected and iron content of SLNs (P=0.07). Increasing magnetic tracer volume and injecting prior to surgery improves transcutaneous 'hotspot' identification but very high volumes, increase the number of nodes excised.
U2 - 10.1016/j.nano.2015.01.010
DO - 10.1016/j.nano.2015.01.010
M3 - Article
C2 - 25680540
SN - 1549-9642
VL - 11
SP - 993
EP - 1002
JO - Nanomedicine : nanotechnology, biology, and medicine
JF - Nanomedicine : nanotechnology, biology, and medicine
IS - 4
ER -