TY - JOUR
T1 - The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells
AU - Xia, Yun
AU - Sancho-Martinez, Ignacio
AU - Nivet, Emmanuel
AU - Rodriguez Esteban, Concepcion
AU - Campistol, Josep Maria
AU - Izpisua Belmonte, Juan Carlos
PY - 2014/11
Y1 - 2014/11
N2 - This protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies.
AB - This protocol presents recently developed methodologies for the differentiation of human pluripotent stem cells (hPSCs) into ureteric bud (UB) progenitor-like cells. Differentiation of human PSCs to UB progenitor-like cells allows for the generation of chimeric kidney cultures in which the human cells can self-assemble into chimeric 3D structures in combination with embryonic mouse kidney cells over a period of 18 d. UB progenitor-like cells are generated by a two-step process that combines in vitro commitment of human PSCs, whether embryonic stem cells (ESCs) or induced PSCs (iPSCs), under chemically defined culture conditions, with ex vivo cultures for the induction of 3D organogenesis. The models described here provide new opportunities for investigating human kidney development, modeling disease, evaluating regenerative medicine strategies, as well as for toxicology studies.
KW - Animals
KW - Cell Culture Techniques
KW - Cell Differentiation
KW - Female
KW - Humans
KW - Kidney
KW - Male
KW - Mice, Inbred Strains
KW - Organ Culture Techniques
KW - Organogenesis
KW - Organoids
KW - Pluripotent Stem Cells
U2 - 10.1038/nprot.2014.182
DO - 10.1038/nprot.2014.182
M3 - Article
C2 - 25340442
SN - 1754-2189
VL - 9
SP - 2693
EP - 2704
JO - Nature Protocols
JF - Nature Protocols
IS - 11
ER -
