Research output per year
Research output per year
Personal profile
Research interests
Dr Kudsiova is currently interested in studying the fate of non-viral gene delivery vectors such as lipid:DNA complexes inside cells. By fluorescently labelling each component of the gene delivery vector with various fluorescent labels, it is possible to track the intracellular fate of the DNA and the lipid over a period of time using confocal fluorescence microscopy.
Recent research has focused on using cationic lipid:DNA complexes incorporating a synthetic bifunctional peptide, pep6, consisting of a Lys16 domain (to bind DNA), and a cyclic domain (CRRETAWAC) known to bind specifically to the cell surface protein, a5b1 intergrin. Incorporation of this ligand was found to greatly improve the transfection efficiency of various lipids in a range of cell lines, and greatly enhance the transport of DNA into the cell nucleus. This work is carried out in collaboration with Dr Hailes and Tabor of the Chemistry Department UCL, and Prof. Ng and Dr. Keppler of the Randall Division of Cell & Molecular Biophysics, KCL.
Other areas of interest:
- Investigating the intracellular uptake and trafficking pathways of lipid:DNA complexes by fluorescently labelling certain cellular compartments and using certain endocytotic pathway inhibitors
- Investigating the physicochemical properties and the phase behaviour of cationic lipid:DNA complexes over a pH range that mimics endosomal conditions
- Investigating the effect of chitosan and chitosan-DNA complexes on epithelial permeability across epithelial cell monolayers
- Structural studies on ternary complexes of DNA-zwitterionic polymer-Ca2+ complexes
Research interests (short)
Non-viral gene delivery vectors such as lipid:DNA complexes inside cells; Investigating the intracellular uptake and trafficking pathways of lipid; Investigating the physicochemical properties and the phase behaviour of cationic lipid; Investigating the effect of chitosan and chitosan-DNA complexes on epithelial permeability across epithelial cell monolayers; Structural studies on ternary complexes of DNA-zwitterionic polymer-Ca2+ complexes
Expertise related to UN Sustainable Development Goals
In 2015, UN member states agreed to 17 global Sustainable Development Goals (SDGs) to end poverty, protect the planet and ensure prosperity for all. This person’s work contributes towards the following SDG(s):
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Collaborations and top research areas from the last five years
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Research output
- 15 Article
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Stability testing of the Pfizer-BioNTech BNT162b2 COVID-19 vaccine: A translational study in UK vaccination centres
Kudsiova, L., Lansley, A., Scutt, G., Allen, M., Bowler, L., Williams, S., Lippett, S., Stafford, S., Tarzi, M., Cross, M. & Okorie, M., 13 Sept 2021, In: BMJ Open Science. 5, 1, e100203.Research output: Contribution to journal › Article › peer-review
Open Access17 Citations (Scopus) -
The discovery and enhanced properties of trichain lipids in lipopolyplex gene delivery systems
Mohammadi, A., Kudsiova, L., Mustapa, M. F. M., Campbell, F., Vlaho, D., Welser, K., Story, H., Tagalakis, A. D., Hart, S. L., Barlow, D. J., Tabor, A. B., Lawrence, M. J. & Hailes, H. C., 23 Jan 2019, In: ORGANIC AND BIOMOLECULAR CHEMISTRY. 17, 4, p. 945-957 13 p.Research output: Contribution to journal › Article › peer-review
Open Access8 Citations (Scopus) -
Trichain cationic lipids: The potential of their lipoplexes for gene delivery
Kudsiova, L., Mohammadi, A., Mustapa, M. F. M., Campbell, F., Welser, K., Vlaho, D., Story, H., Barlow, D. J., Tabor, A. B., Hailes, H. C. & Lawrence, M. J., 18 Dec 2018, In: Biomaterials Science. 7, 1, p. 149-158 10 p.Research output: Contribution to journal › Article › peer-review
Open Access19 Citations (Scopus) -
Delivery of siRNA using ternary complexes containing branched cationic peptides: The role of peptide sequence, branching and targeting
Kudsiova, L., Welser, K., Campbell, F., Mohammadi, A., Dawson, N., Cui, L., Hailes, H. C., Lawrence, M. J. & Tabor, A. B., 1 Mar 2016, In: Molecular Biosystems. 12, 3, p. 934-951 18 p.Research output: Contribution to journal › Article › peer-review
Open AccessFile13 Citations (Scopus)205 Downloads (Pure) -
Gene delivery using ternary lipopolylexes incorporating branched cationic peptides: the role of peptide sequence and branching
Wesler, K., Campbell, F., Kudsiova, L., Mohammadi, A., Dawson, N., Barlow, D. J., Hailes, H., Lawrence, M. J. & Tabor, A., 7 Jan 2013, In: Molecular Pharmaceutics. 10, 1, p. 127-141 15 p., N/A.Research output: Contribution to journal › Article › peer-review
Open Access29 Citations (Scopus)