TY - JOUR
T1 - 3D multicellular systems in disease modelling
T2 - From organoids to organ-on-chip
AU - Goldrick, Caoimhe
AU - Guri, Ina
AU - Herrera-Oropeza, Gabriel
AU - O’Brien-Gore, Charlotte
AU - Roy, Errin
AU - Wojtynska, Maja
AU - Spagnoli, Francesca M.
N1 - Publisher Copyright:
Copyright © 2023 Goldrick, Guri, Herrera-Oropeza, O’Brien-Gore, Roy, Wojtynska and Spagnoli.
PY - 2023/2/2
Y1 - 2023/2/2
N2 - Cell-cell interactions underlay organ formation and function during homeostasis. Changes in communication between cells and their surrounding microenvironment are a feature of numerous human diseases, including metabolic disease and neurological disorders. In the past decade, cross-disciplinary research has been conducted to engineer novel synthetic multicellular organ systems in 3D, including organoids, assembloids, and organ-on-chip models. These model systems, composed of distinct cell types, satisfy the need for a better understanding of complex biological interactions and mechanisms underpinning diseases. In this review, we discuss the emerging field of building 3D multicellular systems and their application for modelling the cellular interactions at play in diseases. We report recent experimental and computational approaches for capturing cell-cell interactions as well as progress in bioengineering approaches for recapitulating these complexities ex vivo. Finally, we explore the value of developing such multicellular systems for modelling metabolic, intestinal, and neurological disorders as major examples of multisystemic diseases, we discuss the advantages and disadvantages of the different approaches and provide some recommendations for further advancing the field.
AB - Cell-cell interactions underlay organ formation and function during homeostasis. Changes in communication between cells and their surrounding microenvironment are a feature of numerous human diseases, including metabolic disease and neurological disorders. In the past decade, cross-disciplinary research has been conducted to engineer novel synthetic multicellular organ systems in 3D, including organoids, assembloids, and organ-on-chip models. These model systems, composed of distinct cell types, satisfy the need for a better understanding of complex biological interactions and mechanisms underpinning diseases. In this review, we discuss the emerging field of building 3D multicellular systems and their application for modelling the cellular interactions at play in diseases. We report recent experimental and computational approaches for capturing cell-cell interactions as well as progress in bioengineering approaches for recapitulating these complexities ex vivo. Finally, we explore the value of developing such multicellular systems for modelling metabolic, intestinal, and neurological disorders as major examples of multisystemic diseases, we discuss the advantages and disadvantages of the different approaches and provide some recommendations for further advancing the field.
KW - assembloids
KW - disease modelling
KW - multicellular systems
KW - organ-on-chip
KW - organoids
UR - http://www.scopus.com/inward/record.url?scp=85148352011&partnerID=8YFLogxK
U2 - 10.3389/fcell.2023.1083175
DO - 10.3389/fcell.2023.1083175
M3 - Review article
AN - SCOPUS:85148352011
SN - 2296-634X
VL - 11
JO - Frontiers in Cell and Developmental Biology
JF - Frontiers in Cell and Developmental Biology
M1 - 1083175
ER -