A guide to super-resolution fluorescence microscopy

Lothar Schermelleh, Rainer Heintzmann, Heinrich Leonhardt

Research output: Contribution to journalLiterature reviewpeer-review

1065 Citations (Scopus)

Abstract

For centuries, cell biology has been based on light microscopy and at the same time been limited by its optical resolution. However, several new technologies have been developed recently that bypass this limit. These new superresolution technologies are either based on tailored illumination, nonlinear fluorophore responses, or the precise localization of single molecules. Overall, these new approaches have created unprecedented new possibilities to investigate the structure and function of cells.
Original languageEnglish
Pages (from-to)165 - 175
Number of pages11
JournalJournal of Cell Biology
Volume190
Issue number2
DOIs
Publication statusPublished - 26 Jul 2010

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