Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets

Ge Zhang; Peter Weinberg; Chris Dunsby; Meng Xing Tang; Sevan Harput; Matthieu Toulemonde; Jacob Broughton-Venner; Jiaqi Zhu; Kai Riemer; Kirsten Christensen-Jeffries; Jemma Brown; Robert J. Eckersley

doi:10.1109/ULTSYM.2019.8926069

Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets

Ge Zhang, Peter Weinberg, Chris Dunsby, Meng Xing Tang, Sevan Harput, Matthieu Toulemonde, Jacob Broughton-Venner, Jiaqi Zhu, Kai Riemer, Kirsten Christensen-Jeffries, Jemma Brown, Robert J. Eckersley

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

7 Citations (Scopus)

Abstract

Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 μm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging.

Original language	English
Title of host publication	2019 IEEE International Ultrasonics Symposium, IUS 2019
Publisher	IEEE Computer Society
Pages	1930-1933
Number of pages	4
Volume	2019-October
ISBN (Electronic)	9781728145969
DOIs	https://doi.org/10.1109/ULTSYM.2019.8926069
Publication status	Published - 1 Oct 2019
Event	2019 IEEE International Ultrasonics Symposium, IUS 2019 - Glasgow, United Kingdom Duration: 6 Oct 2019 → 9 Oct 2019

Conference

Conference	2019 IEEE International Ultrasonics Symposium, IUS 2019
Country/Territory	United Kingdom
City	Glasgow
Period	6/10/2019 → 9/10/2019

Keywords

Decafluorobutane Nanodroplets
Flow Independent
High Frame Rate
Microbubbles
Super-Resolution

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10.1109/ULTSYM.2019.8926069

Cite this

Zhang, G., Weinberg, P., Dunsby, C., Tang, M. X., Harput, S., Toulemonde, M., Broughton-Venner, J., Zhu, J., Riemer, K., Christensen-Jeffries, K., Brown, J., & Eckersley, R. J. (2019). Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets. In 2019 IEEE International Ultrasonics Symposium, IUS 2019 (Vol. 2019-October, pp. 1930-1933). Article 8926069 IEEE Computer Society. https://doi.org/10.1109/ULTSYM.2019.8926069

@inproceedings{ecea1b8e14a3461c82b39121e5a09946,

title = "Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets",

abstract = "Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 μm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging.",

keywords = "Decafluorobutane Nanodroplets, Flow Independent, High Frame Rate, Microbubbles, Super-Resolution",

author = "Ge Zhang and Peter Weinberg and Chris Dunsby and Tang, {Meng Xing} and Sevan Harput and Matthieu Toulemonde and Jacob Broughton-Venner and Jiaqi Zhu and Kai Riemer and Kirsten Christensen-Jeffries and Jemma Brown and Eckersley, {Robert J.}",

year = "2019",

month = oct,

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doi = "10.1109/ULTSYM.2019.8926069",

language = "English",

volume = "2019-October",

pages = "1930--1933",

booktitle = "2019 IEEE International Ultrasonics Symposium, IUS 2019",

publisher = "IEEE Computer Society",

note = "2019 IEEE International Ultrasonics Symposium, IUS 2019 ; Conference date: 06-10-2019 Through 09-10-2019",

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Zhang, G, Weinberg, P, Dunsby, C, Tang, MX, Harput, S, Toulemonde, M, Broughton-Venner, J, Zhu, J, Riemer, K, Christensen-Jeffries, K, Brown, J & Eckersley, RJ 2019, Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets. in 2019 IEEE International Ultrasonics Symposium, IUS 2019. vol. 2019-October, 8926069, IEEE Computer Society, pp. 1930-1933, 2019 IEEE International Ultrasonics Symposium, IUS 2019, Glasgow, United Kingdom, 6/10/2019. https://doi.org/10.1109/ULTSYM.2019.8926069

Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets. / Zhang, Ge; Weinberg, Peter; Dunsby, Chris et al.
2019 IEEE International Ultrasonics Symposium, IUS 2019. Vol. 2019-October IEEE Computer Society, 2019. p. 1930-1933 8926069.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Tang, Meng Xing

AU - Harput, Sevan

AU - Toulemonde, Matthieu

AU - Broughton-Venner, Jacob

AU - Zhu, Jiaqi

AU - Riemer, Kai

AU - Christensen-Jeffries, Kirsten

AU - Brown, Jemma

AU - Eckersley, Robert J.

PY - 2019/10/1

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N2 - Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 μm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging.

AB - Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 μm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging.

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M3 - Conference contribution

AN - SCOPUS:85077540240

VL - 2019-October

SP - 1930

EP - 1933

BT - 2019 IEEE International Ultrasonics Symposium, IUS 2019

PB - IEEE Computer Society

Y2 - 6 October 2019 through 9 October 2019

ER -

Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets

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Keywords

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