@article{65a048bce0044c778990fc4dc609da61,
title = "An in-vitro study investigating the effect of air-abrasion bioactive glasses on dental adhesion, cytotoxicity and odontogenic gene expression",
abstract = "Objective: To assess the microtensile bond strength (MTBS) and interfacial characteristics of universal adhesives applied on dentine air-abraded using different powders. The analysis includes the cytotoxicity of the powders and their effect on odontogenic gene expression. Methods: Sound human dentine specimens were air-abraded using bioglass 45S5 (BAG), polycarboxylated zinc-doped bioglass (SEL), alumina (AL) and submitted to SEM analysis. Resin composite was bonded to air-abraded or smear layer-covered dentine (SML) using an experimental (EXP) or a commercial adhesive (ABU) in etch&rinse (ER) or self-etch (SE) modes. Specimens were stored in artificial saliva (AS) and subjected to MTBS testing after 24 h and 10 months. Interfacial nanoleakage assessment was accomplished using confocal microscopy. The cytotoxicity of the powders was assessed, also the total RNA was extracted and the expression of odontogenic genes was evaluated through RT-PCR. Results: After prolonged AS storage, specimens in the control (SML) and AL groups showed a significant drop in MTBS (p > 0.05), with degradation evident within the bonding interface. Specimens in BAG or SEL air-abraded dentine groups showed no significant difference, with resin-dentine interfaces devoid of important degradation. The metabolic activity of pulp stem cells was not affected by the tested powders. SEL and BAG had no effect on the expression of odontoblast differentiation markers. However, AL particles interfered with the expression of the odontogenic markers. Significance: The use of bioactive glass air-abrasion may prevent severe degradation at the resin-dentine interface. Unlike alumina, bioactive glasses do not interfere with the normal metabolic activity of pulp stem cells and their differentiation to odontoblasts.",
keywords = "Air-abrasion, Biocompatibility, Bonding, Dentine, Odontogenic gene expression, Resin-dentine interface",
author = "Gianrico Spagnuolo and Pires, {Paula Maciel} and Anna Calarco and Gianfranco Peluso and Avijit Banerjee and Sandro Rengo and {Elias Boneta}, {Augusto R.} and Salvatore Sauro",
note = "Funding Information: All authors gave their final approval and agree to be accountable for all aspects of the work. They no conflict of interest with respect to the authorship and/or publication of this paper. All the other materials used in this study were regularly purchased from local distributors, and research facilities were supported by grants “ Ministerio de Ciencia, Innovaci{\'o}n y Universidades ( PID2020-120346GB-I00 ) and Universidad CEU-Cardenal Herrera (Programa FUSP CEU-Santander 2019-2020)” (PI: SS). Paula Maciel Pires was undertaking a PhD exchange program at Cardenal Herrera University during a part of the experimental assay and was supported by a CAPES grant from Brazil (grant numbers 88882.424807/2018-01 and 88881.188518/2018-01 ). The authors also gratefully acknowledge Velopex International, London, UK for a generous donation of the air-abrasion powders used in this study. Funding Information: All authors gave their final approval and agree to be accountable for all aspects of the work. They no conflict of interest with respect to the authorship and/or publication of this paper. All the other materials used in this study were regularly purchased from local distributors, and research facilities were supported by grants ?Ministerio de Ciencia, Innovaci?n y Universidades (PID2020-120346GB-I00) and Universidad CEU-Cardenal Herrera (Programa FUSP CEU-Santander 2019-2020)? (PI: SS). Paula Maciel Pires was undertaking a PhD exchange program at Cardenal Herrera University during a part of the experimental assay and was supported by a CAPES grant from Brazil (grant numbers 88882.424807/2018-01 and 88881.188518/2018-01). The authors also gratefully acknowledge Velopex International, London, UK for a generous donation of the air-abrasion powders used in this study. Publisher Copyright: {\textcopyright} 2021 The Academy of Dental Materials",
year = "2021",
month = nov,
doi = "10.1016/j.dental.2021.09.004",
language = "English",
volume = "37",
pages = "1734--1750",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "ELSEVIER SCI LTD",
number = "11",
}