An iron-regulated ferric reductase associated with the absorption of dietary iron

A T McKie; D Barrow; G O Latunde-Dada; A Rolfs; G Sager; E Mudaly; M Mudaly; C Richardson; D Barlow; A Bomford; T J Peters; K B Raja; S Shirali; M A Hediger; F Farzaneh; R J Simpson

doi:10.1126/science.1057206

An iron-regulated ferric reductase associated with the absorption of dietary iron

A T McKie, D Barrow, G O Latunde-Dada, A Rolfs, G Sager, E Mudaly, M Mudaly, C Richardson, D Barlow, A Bomford, T J Peters, K B Raja, S Shirali, M A Hediger, F Farzaneh, R J Simpson

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Abstract

The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal mucosa. Dcytb shared between 45 and 50% similarity to the cytochrome b561 family of plasma membrane reductases, was highly expressed in the brush-border membrane of duodenal enterocytes, and induced ferric reductase activity when expressed in Xenopus oocytes and cultured cells. Duodenal expression Levels of Dcytb messenger RNA and protein were regulated by changes in physiological modulators of iron absorption. Thus, Dcytb provides an important element in the iron absorption pathway.

Original language	English
Pages (from-to)	1755 - 1759
Number of pages	5
Journal	Science
Volume	291
Issue number	5509
DOIs	https://doi.org/10.1126/science.1057206
Publication status	Published - 2 Mar 2001

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McKie, A. T., Barrow, D., Latunde-Dada, G. O., Rolfs, A., Sager, G., Mudaly, E., Mudaly, M., Richardson, C., Barlow, D., Bomford, A., Peters, T. J., Raja, K. B., Shirali, S., Hediger, M. A., Farzaneh, F., & Simpson, R. J. (2001). An iron-regulated ferric reductase associated with the absorption of dietary iron. Science, 291(5509), 1755 - 1759. https://doi.org/10.1126/science.1057206

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abstract = "The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal mucosa. Dcytb shared between 45 and 50% similarity to the cytochrome b561 family of plasma membrane reductases, was highly expressed in the brush-border membrane of duodenal enterocytes, and induced ferric reductase activity when expressed in Xenopus oocytes and cultured cells. Duodenal expression Levels of Dcytb messenger RNA and protein were regulated by changes in physiological modulators of iron absorption. Thus, Dcytb provides an important element in the iron absorption pathway.",

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AU - Latunde-Dada, G O

AU - Rolfs, A

AU - Sager, G

AU - Mudaly, E

AU - Mudaly, M

AU - Richardson, C

AU - Barlow, D

AU - Bomford, A

AU - Peters, T J

AU - Raja, K B

AU - Shirali, S

AU - Hediger, M A

AU - Farzaneh, F

AU - Simpson, R J

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AB - The ability of intestinal mucosa to absorb dietary ferric iron is attributed to the presence of a brush-border membrane reductase activity that displays adaptive responses to iron status. We have isolated a complementary DNA, Dcytb (for duodenal cytochrome b), which encoded a putative plasma membrane di-heme protein in mouse duodenal mucosa. Dcytb shared between 45 and 50% similarity to the cytochrome b561 family of plasma membrane reductases, was highly expressed in the brush-border membrane of duodenal enterocytes, and induced ferric reductase activity when expressed in Xenopus oocytes and cultured cells. Duodenal expression Levels of Dcytb messenger RNA and protein were regulated by changes in physiological modulators of iron absorption. Thus, Dcytb provides an important element in the iron absorption pathway.

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An iron-regulated ferric reductase associated with the absorption of dietary iron

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