Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection

Gillian Tully; Kevin M. Sullivan; Peter Gill

doi:10.1007/BF00420938

Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection

Gillian Tully^*, Kevin M. Sullivan, Peter Gill

^*Corresponding author for this work

Analytical, Environmental & Forensic Sciences

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

Original language	English
Pages (from-to)	554-562
Number of pages	9
Journal	Human Genetics
Volume	92
Issue number	6
DOIs	https://doi.org/10.1007/BF00420938
Publication status	Published - Dec 1993

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abstract = "The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.",

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AU - Sullivan, Kevin M.

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AB - The polymerase chain reaction was used to amplify six small variable number of tandem repeat loci in two reactions (D19S20 co-amplifying with D17S5 and D1S80; D17S766 co-amplifying with D16S83 and D17S24). When coupled with fluorescent detection of the products, this provides a rapid, highly discriminating automated test. Preferential amplification of small alleles, leading to 'allelic dropout' was found to occur in D19S20 and D16S83. Population databases are presented for Caucasians and Afro-Caribbeans at loci D19S20, D16S83 and D17S24, and for Asians at D19S20.

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Analysis of 6 VNTR loci by 'multiplex' PCR and automated fluorescent detection

Abstract

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