Androgen metabolic response to indomethacin and the alkaline phosphatase inhibitor levamisole in fibroblasts

M Soory, A Tilakaratne

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objectives: The aim of this investigation is to study the effects of indomethacin (I) and the alkaline phosphatase (ALP) inhibitor levamisole (L) on androgen 5alpha-reductase expression in gingival and periosteal fibroblasts, in the context of repair in the periodontium. Chronically inflamed human gingival fibroblasts (HGF) were used to demonstrate the comparative effects of L on HGF and human oral periosteal fibroblasts (HPF). Material and Methods: Monolayer cultures of six cell lines of HPF of the fifth to ninth passage were incubated in duplicate with 14C-testosterone/14C-4-androstenedione as substrates in Eagle's MEM; I was added at concentrations of 1 and 3 mug/ml in the presence or absence of the established inhibitory concentration of 30 mug/ml L and incubated for 24 h. The medium was solvent extracted for radioactive metabolites, separated by thin layer chromatography and quantified. Results: L caused 50% inhibition of 5alpha-reductase and 17beta-hydroxysteroid dehydrogenase activity in HGF. In HPF, 5alpha-reductase expression was enhanced by I with both substrates, by 65-76% (n=6; p
Original languageEnglish
Pages (from-to)1069 - 1074
Number of pages6
JournalJournal of Clinical Periodontology
Volume30
Issue number12
DOIs
Publication statusPublished - Dec 2003

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