TY - JOUR
T1 - Androgen metabolic response to indomethacin and the alkaline phosphatase inhibitor levamisole in fibroblasts
AU - Soory, M
AU - Tilakaratne, A
PY - 2003/12
Y1 - 2003/12
N2 - Objectives: The aim of this investigation is to study the effects of indomethacin (I) and the alkaline phosphatase (ALP) inhibitor levamisole (L) on androgen 5alpha-reductase expression in gingival and periosteal fibroblasts, in the context of repair in the periodontium. Chronically inflamed human gingival fibroblasts (HGF) were used to demonstrate the comparative effects of L on HGF and human oral periosteal fibroblasts (HPF). Material and Methods: Monolayer cultures of six cell lines of HPF of the fifth to ninth passage were incubated in duplicate with 14C-testosterone/14C-4-androstenedione as substrates in Eagle's MEM; I was added at concentrations of 1 and 3 mug/ml in the presence or absence of the established inhibitory concentration of 30 mug/ml L and incubated for 24 h. The medium was solvent extracted for radioactive metabolites, separated by thin layer chromatography and quantified. Results: L caused 50% inhibition of 5alpha-reductase and 17beta-hydroxysteroid dehydrogenase activity in HGF. In HPF, 5alpha-reductase expression was enhanced by I with both substrates, by 65-76% (n=6; p
AB - Objectives: The aim of this investigation is to study the effects of indomethacin (I) and the alkaline phosphatase (ALP) inhibitor levamisole (L) on androgen 5alpha-reductase expression in gingival and periosteal fibroblasts, in the context of repair in the periodontium. Chronically inflamed human gingival fibroblasts (HGF) were used to demonstrate the comparative effects of L on HGF and human oral periosteal fibroblasts (HPF). Material and Methods: Monolayer cultures of six cell lines of HPF of the fifth to ninth passage were incubated in duplicate with 14C-testosterone/14C-4-androstenedione as substrates in Eagle's MEM; I was added at concentrations of 1 and 3 mug/ml in the presence or absence of the established inhibitory concentration of 30 mug/ml L and incubated for 24 h. The medium was solvent extracted for radioactive metabolites, separated by thin layer chromatography and quantified. Results: L caused 50% inhibition of 5alpha-reductase and 17beta-hydroxysteroid dehydrogenase activity in HGF. In HPF, 5alpha-reductase expression was enhanced by I with both substrates, by 65-76% (n=6; p
UR - http://www.scopus.com/inward/record.url?scp=0348147431&partnerID=8YFLogxK
U2 - 10.1046/j.0303-6979.2003.00428.x
DO - 10.1046/j.0303-6979.2003.00428.x
M3 - Article
SN - 1600-051X
VL - 30
SP - 1069
EP - 1074
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 12
ER -