TY - JOUR
T1 - Antibiotic-in-cyclodextrin-in-liposomes
T2 - formulation development and interactions with model bacterial membranes
AU - Vandera, Kalliopi-Kelli A.
AU - Picconi, Pietro
AU - Valero, Margarita
AU - González-Gaitano, Gustavo
AU - Woods, Arcadia
AU - Zain, Nur Masirah
AU - Bruce, Kenneth
AU - Clifton, Luke A.
AU - Skoda, Maximilian W.A.
AU - Rahman, Khondaker Miraz
AU - Harvey, Richard D.
AU - Dreiss, Cecile
PY - 2020/7/6
Y1 - 2020/7/6
N2 - Gram-negative bacteria possess numerous defenses against antibiotics, due to the intrinsic permeability barrier of their outer membrane (OM), explaining the recalcitrance of some common and life-threatening infections. We report the formulation of a new drug, PPA148, which shows promising activity against all Gram-negative bacteria included in the ESKAPEE pathogens. PPA148 was solubilized by inclusion complexation with cyclodextrin followed by encapsulation in liposomes. The complex and liposomal formulation presented increased activity against E. coli compared to the pure drug when assessed with the Kirby Bauer assay. The novel formulation containing 1 μg PPA148 reached similar efficacy levels equivalent to those of 30 μg of pure rifampicin. A range of biophysical techniques was used to explore the mechanism of drug uptake. Langmuir trough (LT) and neutron reflectivity (NR) techniques were employed to monitor the interactions between the drug and the formulation with model membranes. We found evidence for liposome fusion with the model Gram-negative outer membrane and for cyclodextrins acting as inner membrane (IM) permeation enhancers without presenting intrinsic antimicrobial activity. An antibiotic-in-cyclodextrin-in-liposomes (ACL) formulation was developed, which targets both the bacterial OM and IM, and offers promise as a means to breach the Gram-negative cell envelope.
AB - Gram-negative bacteria possess numerous defenses against antibiotics, due to the intrinsic permeability barrier of their outer membrane (OM), explaining the recalcitrance of some common and life-threatening infections. We report the formulation of a new drug, PPA148, which shows promising activity against all Gram-negative bacteria included in the ESKAPEE pathogens. PPA148 was solubilized by inclusion complexation with cyclodextrin followed by encapsulation in liposomes. The complex and liposomal formulation presented increased activity against E. coli compared to the pure drug when assessed with the Kirby Bauer assay. The novel formulation containing 1 μg PPA148 reached similar efficacy levels equivalent to those of 30 μg of pure rifampicin. A range of biophysical techniques was used to explore the mechanism of drug uptake. Langmuir trough (LT) and neutron reflectivity (NR) techniques were employed to monitor the interactions between the drug and the formulation with model membranes. We found evidence for liposome fusion with the model Gram-negative outer membrane and for cyclodextrins acting as inner membrane (IM) permeation enhancers without presenting intrinsic antimicrobial activity. An antibiotic-in-cyclodextrin-in-liposomes (ACL) formulation was developed, which targets both the bacterial OM and IM, and offers promise as a means to breach the Gram-negative cell envelope.
KW - Langmuir monolayers
KW - antibiotic formulation
KW - antimicrobial resistance
KW - asymmetric bilayer
KW - membrane fusion
KW - neutron reflectivity
UR - http://www.scopus.com/inward/record.url?scp=85087533997&partnerID=8YFLogxK
U2 - 10.1021/acs.molpharmaceut.0c00096
DO - 10.1021/acs.molpharmaceut.0c00096
M3 - Article
SN - 1543-8384
VL - 17
SP - 2354
EP - 2369
JO - Molecular Pharmaceutics
JF - Molecular Pharmaceutics
IS - 7
ER -