Artifact-free high-density localization microscopy analysis

Richard J. Marsh; Karin Pfisterer; Pauline Bennett; Liisa M Hirvonen; Mathias Gautel; Gareth E. Jones; Susan Cox

doi:10.1038/s41592-018-0072-5

Artifact-free high-density localization microscopy analysis

Richard J. Marsh, Karin Pfisterer, Pauline Bennett, Liisa M Hirvonen, Mathias Gautel, Gareth E. Jones, Susan Cox

King's College London

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Abstract

High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

Original language	English
Pages (from-to)	689-692
Journal	NATURE METHODS
Volume	15
Early online date	30 Jul 2018
DOIs	https://doi.org/10.1038/s41592-018-0072-5
Publication status	Published - Sept 2018

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Artefact-free high density_MARSH_Accepted8June2018_GREEN AAMAccepted author manuscript, 637 KB
Artefact-free high density SUPP INFO_MARSH_Accepted8June2018_GREEN AAMAccepted author manuscript, 3.07 MB

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title = "Artifact-free high-density localization microscopy analysis",

abstract = "High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.",

author = "Marsh, {Richard J.} and Karin Pfisterer and Pauline Bennett and Hirvonen, {Liisa M} and Mathias Gautel and Jones, {Gareth E.} and Susan Cox",

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AU - Hirvonen, Liisa M

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AU - Jones, Gareth E.

AU - Cox, Susan

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AB - High-density analysis methods for localization microscopy increase acquisition speed but produce artifacts. We demonstrate that these artifacts can be eliminated by the combination of Haar wavelet kernel (HAWK) analysis with standard single-frame fitting. We tested the performance of this method on synthetic, fixed-cell, and live-cell data, and found that HAWK preprocessing yielded reconstructions that reflected the structure of the sample, thus enabling high-speed, artifact-free super-resolution imaging of live cells.

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DO - 10.1038/s41592-018-0072-5

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VL - 15

SP - 689

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JO - NATURE METHODS

JF - NATURE METHODS

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Artifact-free high-density localization microscopy analysis

Abstract

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