Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity

J Downes; B Olsvik; S J Hiom; D A Spratt; S L Cheeseman; I Olsen; A J Weightman; W G Wade

Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity

J Downes, B Olsvik, S J Hiom, D A Spratt, S L Cheeseman, I Olsen, A J Weightman, W G Wade

King's College London

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Five strains of anaerobic non-sporing Cram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess tone strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they were found to constitute a novel branch of the low G+C Cram-positive division of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Holdemania filiformis. A new genus Bulleidia, and the species Bulleidia extructa, are proposed. Growth of B. extructa in broth media was poor but was enhanced by the addition of fructose, glucose or maltose together with Tween 80. Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fermentation. The G+C content of the DNA of the type strain is 38 mol%. The type strain of Bulleidia extructa is DSM 13220(T).

Original language	English
Pages (from-to)	979 - 983
Number of pages	5
Journal	INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
Volume	50
Publication status	Published - 2000

Cite this

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title = "Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity",

abstract = "Five strains of anaerobic non-sporing Cram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess tone strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they were found to constitute a novel branch of the low G+C Cram-positive division of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Holdemania filiformis. A new genus Bulleidia, and the species Bulleidia extructa, are proposed. Growth of B. extructa in broth media was poor but was enhanced by the addition of fructose, glucose or maltose together with Tween 80. Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fermentation. The G+C content of the DNA of the type strain is 38 mol%. The type strain of Bulleidia extructa is DSM 13220(T).",

author = "J Downes and B Olsvik and Hiom, {S J} and Spratt, {D A} and Cheeseman, {S L} and I Olsen and Weightman, {A J} and Wade, {W G}",

year = "2000",

language = "English",

volume = "50",

pages = "979 -- 983",

journal = "INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY",

publisher = "Society for General Microbiology",

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TY - JOUR

T1 - Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity

AU - Downes, J

AU - Olsvik, B

AU - Hiom, S J

AU - Spratt, D A

AU - Cheeseman, S L

AU - Olsen, I

AU - Weightman, A J

AU - Wade, W G

PY - 2000

Y1 - 2000

N2 - Five strains of anaerobic non-sporing Cram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess tone strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they were found to constitute a novel branch of the low G+C Cram-positive division of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Holdemania filiformis. A new genus Bulleidia, and the species Bulleidia extructa, are proposed. Growth of B. extructa in broth media was poor but was enhanced by the addition of fructose, glucose or maltose together with Tween 80. Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fermentation. The G+C content of the DNA of the type strain is 38 mol%. The type strain of Bulleidia extructa is DSM 13220(T).

AB - Five strains of anaerobic non-sporing Cram-positive bacilli isolated from advanced periodontitis (four strains) and a dentoalveolar abscess tone strain) that did not correspond to existing species were subjected to phenotypic and genetic characterization. Following 16S rDNA sequence analysis, they were found to constitute a novel branch of the low G+C Cram-positive division of the phylogenetic tree related to Erysipelothrix rhusiopathiae and Holdemania filiformis. A new genus Bulleidia, and the species Bulleidia extructa, are proposed. Growth of B. extructa in broth media was poor but was enhanced by the addition of fructose, glucose or maltose together with Tween 80. Glucose and maltose were fermented and arginine was hydrolysed. Acetate, lactate and trace amounts of succinate were the end products of glucose fermentation. The G+C content of the DNA of the type strain is 38 mol%. The type strain of Bulleidia extructa is DSM 13220(T).

M3 - Article

VL - 50

SP - 979

EP - 983

JO - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

JF - INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

ER -

Bulleidia extructa gen. nov., sp nov., isolated from the oval cavity

Abstract

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