Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

Salvatore Sauro; Ashvin Babbar; Borzo Gharibi; Victor  Feitosa; Ricardo Carvalho; Lidiany Rodrigues; Avijit Banerjee; Timothy F Watson

doi:10.1016/j.dental.2018.02.008

Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

Salvatore Sauro, Ashvin Babbar, Borzo Gharibi, Victor Feitosa, Ricardo Carvalho, Lidiany Rodrigues, Avijit Banerjee, Timothy F Watson

Centre for Oral, Clinical & Translational Sciences

CEU Cardenal Herrera University

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

430 Downloads (Pure)

Abstract

Objective

Materials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.

Methods

Four calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24 h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).

Results

The lowest FS and FT at 24 h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p < 0.05).

Significance

The innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.

Original language	English
Number of pages	11
Journal	Dental Materials
Early online date	16 Mar 2018
DOIs	https://doi.org/10.1016/j.dental.2018.02.008
Publication status	E-pub ahead of print - 16 Mar 2018

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10.1016/j.dental.2018.02.008

Cellular differentiation, bioactive and_SAURO_Publishedonline16March2018_GREEN AAM (CC BY-NC-ND)Accepted author manuscript, 4.34 MBLicence: CC BY-NC-ND

Cite this

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title = "Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials",

abstract = "ObjectiveMaterials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.MethodsFour calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24 h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).ResultsThe lowest FS and FT at 24 h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p < 0.05).SignificanceThe innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.",

author = "Salvatore Sauro and Ashvin Babbar and Borzo Gharibi and Victor Feitosa and Ricardo Carvalho and Lidiany Rodrigues and Avijit Banerjee and Watson, {Timothy F}",

year = "2018",

month = mar,

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doi = "10.1016/j.dental.2018.02.008",

language = "English",

journal = "Dental Materials",

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TY - JOUR

T1 - Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

AU - Sauro, Salvatore

AU - Babbar, Ashvin

AU - Gharibi, Borzo

AU - Feitosa, Victor

AU - Carvalho, Ricardo

AU - Rodrigues, Lidiany

AU - Banerjee, Avijit

AU - Watson, Timothy F

PY - 2018/3/16

Y1 - 2018/3/16

N2 - ObjectiveMaterials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.MethodsFour calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24 h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).ResultsThe lowest FS and FT at 24 h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p < 0.05).SignificanceThe innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.

AB - ObjectiveMaterials for pulp protection should have therapeutic properties in order to stimulate remineralization and pulp reparative processes. The aim of this study was to evaluate the mechanical properties, biocompatibility, cell differentiation and bioactivity of experimental light-curable resin-based materials containing bioactive micro-fillers.MethodsFour calcium-phosphosilicate micro-fillers were prepared and incorporated into a resin blend: 1) Bioglass 45S5 (BAG); 2) zinc-doped bioglass (BAG-Zn); 3) βTCP-modified calcium silicate (β-CS); 4) zinc-doped β-CS (β-CS-Zn). These experimental resins were tested for flexural strength (FS) and fracture toughness (FT) after 24 h and 30-day storage in simulated body fluid (SBF). Cytotoxicity was evaluated using MTT assay, while bioactivity was evaluated using mineralization and gene expression assays (Runx-2 & ALP).ResultsThe lowest FS and FT at 24 h was attained with β-CS resin, while all the other tested materials exhibited a decrease in FS after prolonged storage in SBF. β-CS-Zn maintained a stable FT after 30-day SBF aging. Incorporation of bioactive micro-fillers had no negative effect on the biocompatibility of the experimental materials tested in this study. The inclusion of zinc-doped fillers significantly increased the cellular remineralization potential and expression of the osteogenic genes Runx2 and ALP (p < 0.05).SignificanceThe innovative materials tested in this study, in particular those containing β-CS-Zn and BAG-Zn may promote cell differentiation and mineralization. Thus, these materials might represent suitable therapeutic pulp protection materials for minimally invasive and atraumatic restorative treatments.

U2 - 10.1016/j.dental.2018.02.008

DO - 10.1016/j.dental.2018.02.008

M3 - Article

SN - 0109-5641

JO - Dental Materials

JF - Dental Materials

ER -

Cellular differentiation, bioactive and mechanical properties of experimental light-curing pulp protection materials

Abstract

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