Colorimetric determination of L-phenylacetyl carbinol produced by biotransformation of benzaldehyde and pyruvate using Saccharomyces cerevisiae

P. Nikolova; A. Long; O. P. Ward

doi:10.1007/BF00152751

Colorimetric determination of L-phenylacetyl carbinol produced by biotransformation of benzaldehyde and pyruvate using Saccharomyces cerevisiae

P. Nikolova, A. Long, O. P. Ward

Institute of Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The utility of a colorimetric method, based on the Voges-Proskauer reaction, for L-phenylacetyl carbinol (L-PAC) determination has been evaluated. The relative absorbances observed for equivalent concentrations of L-PAC and acetylbenzoyl at 580 nm were determined. L-PAC manifested a lower absorbance (60–68%) than the equivalent acetylbenzoyl concentration.

Original language	English
Article number	N/A
Pages (from-to)	31-34
Number of pages	4
Journal	BIOTECHNOLOGY TECHNIQUES
Volume	5
Issue number	1
DOIs	https://doi.org/10.1007/BF00152751
Publication status	Published - 1 Jan 1991

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10.1007/BF00152751

Cite this

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title = "Colorimetric determination of L-phenylacetyl carbinol produced by biotransformation of benzaldehyde and pyruvate using Saccharomyces cerevisiae",

abstract = "The utility of a colorimetric method, based on the Voges-Proskauer reaction, for L-phenylacetyl carbinol (L-PAC) determination has been evaluated. The relative absorbances observed for equivalent concentrations of L-PAC and acetylbenzoyl at 580 nm were determined. L-PAC manifested a lower absorbance (60–68%) than the equivalent acetylbenzoyl concentration.",

author = "P. Nikolova and A. Long and Ward, {O. P.}",

year = "1991",

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AU - Long, A.

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N2 - The utility of a colorimetric method, based on the Voges-Proskauer reaction, for L-phenylacetyl carbinol (L-PAC) determination has been evaluated. The relative absorbances observed for equivalent concentrations of L-PAC and acetylbenzoyl at 580 nm were determined. L-PAC manifested a lower absorbance (60–68%) than the equivalent acetylbenzoyl concentration.

AB - The utility of a colorimetric method, based on the Voges-Proskauer reaction, for L-phenylacetyl carbinol (L-PAC) determination has been evaluated. The relative absorbances observed for equivalent concentrations of L-PAC and acetylbenzoyl at 580 nm were determined. L-PAC manifested a lower absorbance (60–68%) than the equivalent acetylbenzoyl concentration.

U2 - 10.1007/BF00152751

DO - 10.1007/BF00152751

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