Conversion of human fibroblasts to angioblast-like progenitor cells

Leo Kurian; Ignacio Sancho-Martinez; Emmanuel Nivet; Aitor Aguirre; Krystal Moon; Caroline Pendaries; Cecile Volle-Challier; Francoise Bono; Jean-Marc Herbert; Julian Pulecio; Yun Xia; Mo Li; Nuria Montserrat; Sergio Ruiz; Ilir Dubova; Concepcion Rodriguez; Ahmet M Denli; Francesca S Boscolo; Rathi D Thiagarajan; Fred H Gage; Jeanne F Loring; Louise C Laurent; Juan Carlos Izpisua Belmonte

doi:10.1038/nmeth.2255

Conversion of human fibroblasts to angioblast-like progenitor cells

Leo Kurian, Ignacio Sancho-Martinez, Emmanuel Nivet, Aitor Aguirre, Krystal Moon, Caroline Pendaries, Cecile Volle-Challier, Francoise Bono, Jean-Marc Herbert, Julian Pulecio, Yun Xia, Mo Li, Nuria Montserrat, Sergio Ruiz, Ilir Dubova, Concepcion Rodriguez, Ahmet M Denli, Francesca S Boscolo, Rathi D Thiagarajan, Fred H GageJeanne F Loring, Louise C Laurent, Juan Carlos Izpisua Belmonte

Centre for Stem Cells & Regenerative Medicine

Research output: Contribution to journal › Article › peer-review

136 Citations (Scopus)

Abstract

Lineage conversion of one somatic cell type to another is an attractive approach for generating specific human cell types. Lineage conversion can be direct, in the absence of proliferation and multipotent progenitor generation, or indirect, by the generation of expandable multipotent progenitor states. We report the development of a reprogramming methodology in which cells transition through a plastic intermediate state, induced by brief exposure to reprogramming factors, followed by differentiation. We use this approach to convert human fibroblasts to mesodermal progenitor cells, including by non-integrative approaches. These progenitor cells demonstrated bipotent differentiation potential and could generate endothelial and smooth muscle lineages. Differentiated endothelial cells exhibited neo-angiogenesis and anastomosis in vivo. This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies.

Original language	English
Pages (from-to)	77-83
Number of pages	7
Journal	NATURE METHODS
Volume	10
Issue number	1
Early online date	2 Dec 2012
DOIs	https://doi.org/10.1038/nmeth.2255
Publication status	Published - Jan 2013

Keywords

Animals
Biomarkers
Blotting, Western
Cell Differentiation
Cell Lineage
Cell Movement
Cell Proliferation
Cells, Cultured
Cellular Reprogramming
Endothelium, Vascular
Fibroblasts
Flow Cytometry
Fluorescent Antibody Technique
Humans
Mice
Myocytes, Smooth Muscle
Neovascularization, Physiologic
RNA, Messenger
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells

Access to Document

10.1038/nmeth.2255

Cite this

Kurian, L., Sancho-Martinez, I., Nivet, E., Aguirre, A., Moon, K., Pendaries, C., Volle-Challier, C., Bono, F., Herbert, J.-M., Pulecio, J., Xia, Y., Li, M., Montserrat, N., Ruiz, S., Dubova, I., Rodriguez, C., Denli, A. M., Boscolo, F. S., Thiagarajan, R. D., ... Izpisua Belmonte, J. C. (2013). Conversion of human fibroblasts to angioblast-like progenitor cells. NATURE METHODS, 10(1), 77-83. https://doi.org/10.1038/nmeth.2255

@article{9a8a3a18750f4885b518a60f7940cacb,

title = "Conversion of human fibroblasts to angioblast-like progenitor cells",

abstract = "Lineage conversion of one somatic cell type to another is an attractive approach for generating specific human cell types. Lineage conversion can be direct, in the absence of proliferation and multipotent progenitor generation, or indirect, by the generation of expandable multipotent progenitor states. We report the development of a reprogramming methodology in which cells transition through a plastic intermediate state, induced by brief exposure to reprogramming factors, followed by differentiation. We use this approach to convert human fibroblasts to mesodermal progenitor cells, including by non-integrative approaches. These progenitor cells demonstrated bipotent differentiation potential and could generate endothelial and smooth muscle lineages. Differentiated endothelial cells exhibited neo-angiogenesis and anastomosis in vivo. This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies.",

keywords = "Animals, Biomarkers, Blotting, Western, Cell Differentiation, Cell Lineage, Cell Movement, Cell Proliferation, Cells, Cultured, Cellular Reprogramming, Endothelium, Vascular, Fibroblasts, Flow Cytometry, Fluorescent Antibody Technique, Humans, Mice, Myocytes, Smooth Muscle, Neovascularization, Physiologic, RNA, Messenger, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Stem Cells",

author = "Leo Kurian and Ignacio Sancho-Martinez and Emmanuel Nivet and Aitor Aguirre and Krystal Moon and Caroline Pendaries and Cecile Volle-Challier and Francoise Bono and Jean-Marc Herbert and Julian Pulecio and Yun Xia and Mo Li and Nuria Montserrat and Sergio Ruiz and Ilir Dubova and Concepcion Rodriguez and Denli, {Ahmet M} and Boscolo, {Francesca S} and Thiagarajan, {Rathi D} and Gage, {Fred H} and Loring, {Jeanne F} and Laurent, {Louise C} and {Izpisua Belmonte}, {Juan Carlos}",

year = "2013",

month = jan,

doi = "10.1038/nmeth.2255",

language = "English",

volume = "10",

pages = "77--83",

journal = "NATURE METHODS",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "1",

}

Kurian, L, Sancho-Martinez, I, Nivet, E, Aguirre, A, Moon, K, Pendaries, C, Volle-Challier, C, Bono, F, Herbert, J-M, Pulecio, J, Xia, Y, Li, M, Montserrat, N, Ruiz, S, Dubova, I, Rodriguez, C, Denli, AM, Boscolo, FS, Thiagarajan, RD, Gage, FH, Loring, JF, Laurent, LC & Izpisua Belmonte, JC 2013, 'Conversion of human fibroblasts to angioblast-like progenitor cells', NATURE METHODS, vol. 10, no. 1, pp. 77-83. https://doi.org/10.1038/nmeth.2255

TY - JOUR

T1 - Conversion of human fibroblasts to angioblast-like progenitor cells

AU - Kurian, Leo

AU - Sancho-Martinez, Ignacio

AU - Nivet, Emmanuel

AU - Aguirre, Aitor

AU - Moon, Krystal

AU - Pendaries, Caroline

AU - Volle-Challier, Cecile

AU - Bono, Francoise

AU - Herbert, Jean-Marc

AU - Pulecio, Julian

AU - Xia, Yun

AU - Li, Mo

AU - Montserrat, Nuria

AU - Ruiz, Sergio

AU - Dubova, Ilir

AU - Rodriguez, Concepcion

AU - Denli, Ahmet M

AU - Boscolo, Francesca S

AU - Thiagarajan, Rathi D

AU - Gage, Fred H

AU - Loring, Jeanne F

AU - Laurent, Louise C

AU - Izpisua Belmonte, Juan Carlos

PY - 2013/1

Y1 - 2013/1

N2 - Lineage conversion of one somatic cell type to another is an attractive approach for generating specific human cell types. Lineage conversion can be direct, in the absence of proliferation and multipotent progenitor generation, or indirect, by the generation of expandable multipotent progenitor states. We report the development of a reprogramming methodology in which cells transition through a plastic intermediate state, induced by brief exposure to reprogramming factors, followed by differentiation. We use this approach to convert human fibroblasts to mesodermal progenitor cells, including by non-integrative approaches. These progenitor cells demonstrated bipotent differentiation potential and could generate endothelial and smooth muscle lineages. Differentiated endothelial cells exhibited neo-angiogenesis and anastomosis in vivo. This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies.

AB - Lineage conversion of one somatic cell type to another is an attractive approach for generating specific human cell types. Lineage conversion can be direct, in the absence of proliferation and multipotent progenitor generation, or indirect, by the generation of expandable multipotent progenitor states. We report the development of a reprogramming methodology in which cells transition through a plastic intermediate state, induced by brief exposure to reprogramming factors, followed by differentiation. We use this approach to convert human fibroblasts to mesodermal progenitor cells, including by non-integrative approaches. These progenitor cells demonstrated bipotent differentiation potential and could generate endothelial and smooth muscle lineages. Differentiated endothelial cells exhibited neo-angiogenesis and anastomosis in vivo. This methodology for indirect lineage conversion to angioblast-like cells adds to the armamentarium of reprogramming approaches aimed at the study and treatment of ischemic pathologies.

KW - Animals

KW - Biomarkers

KW - Blotting, Western

KW - Cell Differentiation

KW - Cell Lineage

KW - Cell Movement

KW - Cell Proliferation

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - Endothelium, Vascular

KW - Fibroblasts

KW - Flow Cytometry

KW - Fluorescent Antibody Technique

KW - Humans

KW - Mice

KW - Myocytes, Smooth Muscle

KW - Neovascularization, Physiologic

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Stem Cells

U2 - 10.1038/nmeth.2255

DO - 10.1038/nmeth.2255

M3 - Article

C2 - 23202434

SN - 1548-7091

VL - 10

SP - 77

EP - 83

JO - NATURE METHODS

JF - NATURE METHODS

IS - 1

ER -

Conversion of human fibroblasts to angioblast-like progenitor cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this