@article{cbb6c5beeee24c278ff53ab890658266,
title = "Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease",
abstract = "Human oral soft tissues provide the first barrier of defence against chronic inflammatory disease and hold a remarkable scarless wounding phenotype. Tissue homeostasis requires coordinated actions of epithelial, mesenchymal, and immune cells. However, the extent of heterogeneity within the human oral mucosa and how tissue cell types are affected during the course of disease progression is unknown. Using single-cell transcriptome profiling we reveal za striking remodelling of the epithelial and mesenchymal niches with a decrease in functional populations that are linked to the aetiology of the disease. Analysis of ligand–receptor interaction pairs identify potential intercellular hubs driving the inflammatory component of the disease. Our work establishes a reference map of the human oral mucosa in health and disease, and a framework for the development of new therapeutic strategies.",
author = "Ana Caetano and Val Yianni and Volponi, {Ana Angelova} and Veronica Booth and Eleanor D'Agostino and Paul Sharpe",
note = "Funding Information: We thank all the patients who contributed to this study, the support of our Periodontology MClinD-ent students, GSTT nursing staff and Dr Pegah Heidarzadeh Pasha at Guy{\textquoteright}s Hospital. We thank all CCRB laboratory technicians for their support, especially Dr Alasdair Edgar for tissue processing and H and E staining, and Dr Susmitha Rao for tissue culture. We acknowledge support of the BRC Flow Cytometry and BRC Genomics cores at Guy{\textquoteright}s Hospital for their services. We thank Dr Ranjit Bhogal, Dr Fei Ling Lim, Miss Alison Russell and Dr Jenny Pople for their support at Unilever. The research described was supported by the BBSRC Industrial CASE Studentship (Grant Ref: BB/P504506/1) and National Institute for Health Research{\textquoteright}s Biomedical Research Centre based at Guy{\textquoteright}s and St Thomas{\textquoteright} NHS Foundation Trust and King{\textquoteright}s College London. The views expressed are those of the authors and not necessarily those of the NHS, the National Institute for Health Research, or the Department of Health. This work was funded by Unilever in the form of a research grant awarded to PTS. Funding Information: We thank all the patients who contributed to this study, the support of our Periodontology MClinD-ent students, GSTT nursing staff and Dr Pegah Heidarzadeh Pasha at Guy?s Hospital. We thank all CCRB laboratory technicians for their support, especially Dr Alasdair Edgar for tissue processing and H and E staining, and Dr Susmitha Rao for tissue culture. We acknowledge support of the BRC Flow Cytometry and BRC Genomics cores at Guy?s Hospital for their services. We thank Dr Ranjit Bhogal, Dr Fei Ling Lim, Miss Alison Russell and Dr Jenny Pople for their support at Unilever. The research described was supported by the BBSRC Industrial CASE Studentship (Grant Ref: BB/P504506/1) and National Institute for Health Research?s Biomedical Research Centre based at Guy?s and St Thomas? NHS Foundation Trust and King?s College London. The views expressed are those of the authors and not necessarily those of the NHS, the National Institute for Health Research, or the Department of Health. This work was funded by Unilever in the form of a research grant awarded to PTS. Publisher Copyright: {\textcopyright} Caetano et al. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = jan,
day = "4",
doi = "10.7554/eLife.62810",
language = "English",
volume = "10",
pages = "1--23",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications, Ltd",
}
