Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

J R Marchesi; T Sato; A J Weightman; T A Martin; J C Fry; S J Hiom; D Dymock; W G Wade

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

J R Marchesi, T Sato, A J Weightman, T A Martin, J C Fry, S J Hiom, D Dymock, W G Wade

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Abstract

We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

Original language	English
Pages (from-to)	795-9
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	64
Issue number	2
Publication status	Published - 1998

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC106123/

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title = "Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA",

abstract = "We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.",

author = "Marchesi, {J R} and T Sato and Weightman, {A J} and Martin, {T A} and Fry, {J C} and Hiom, {S J} and D Dymock and Wade, {W G}",

year = "1998",

language = "English",

volume = "64",

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AU - Marchesi, J R

AU - Sato, T

AU - Weightman, A J

AU - Martin, T A

AU - Fry, J C

AU - Hiom, S J

AU - Dymock, D

AU - Wade, W G

PY - 1998

Y1 - 1998

N2 - We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

AB - We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

M3 - Article

C2 - 9464425

SN - 0099-2240

VL - 64

SP - 795

EP - 799

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -

Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA

Abstract

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