@article{3d5884df0f23416295f554c356765c42,
title = "Development of a high-speed line-scanning fluorescence lifetime imaging microscope for biological imaging",
abstract = "We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.",
author = "Hanning Mai and Anneliese Jarman and Erdogan, {Ahmet T.} and Conor Treacy and Neil Finlayson and Henderson, {Robert K.} and Poland, {Simon P.}",
note = "Funding Information: UK Research and Innovation (MR/T04067X/1); Engineering and Physical Sciences Research Council (EP/K03197X/1); Royal Society (IES\R3\183065). Funding Information: Acknowledgments. This work was supported by a UKRI Future Leaders Fellowship. We would also like to thank ST Microelectronics, Imaging Division, Edinburgh, for their support in manufacturing the CMOS SPAD line array. We acknowledge the help and support of Prof. Simon Ameer-Beg and the Comprehensive Cancer Centre at King{\textquoteright}s College London for providing equipment used in this research. Publisher Copyright: {\textcopyright} 2023 Optica Publishing Group.",
year = "2023",
month = apr,
day = "15",
doi = "10.1364/OL.482403",
language = "English",
volume = "48",
pages = "2042--2045",
journal = "Optics letters",
issn = "1539-4794",
publisher = "Optica",
number = "8",
}