TY - JOUR
T1 - Development of a rapid and sensitive method for determination of cysteine/cystine ratio in chemically defined media
AU - Alwael, Hassan
AU - Connolly, Damian
AU - Barron, Leon
AU - Paull, Brett
PY - 2010/6/11
Y1 - 2010/6/11
N2 - Two rapid, sensitive and quantitative methods for the determination of the cysteine and cystine ratio in complex defined media feedstock using monolithic reversed-phase liquid chromatography (RPLC) and RPLC-MS are presented. Cysteine is pre-derivatised with purified 2-chloro-1-methylquinolinium tetrafluoroborate (CMQT) and separated from other derivatisation products on a narrow-bore 50 mm x 2 mm I.D. monolithic C-18 column with UV detection at 355 nm. For reversed-phase LC (RPLC) the separation is carried out isocratically using a mobile phase of 50 mM trichloroacetic acid (TCA) adjusted to pH 2.5 with lithium hydroxide (LiOH) and acetonitrile (83:14) pumped at 1.5 mL/min with an elevated column temperature. For RPLC-MS an ammonium acetate and acetonitrile gradient method was developed with a reduced flow rate of 0.3 mL/min. The treatment of the samples consisted of dividing them into two aliquots, the first aliquot is analysed for cysteine and the second aliquot is analysed for cystine after its quantitative reduction to cysteine using tris(2-carboxyethyl)phosphine (TCEP). Both methods are linear, with R-2 > 0.999 for 0.25-500 mu M for cysteine and 0.25-250 mu M for cystine using the LC-UV method, sensitive, with detection limit of 36 nM for cysteine, and precise, with <= 1.1% RSD for both retention time and peak area (n = 6). Samples (n = 31) of an industry standard and supplied chemically defined media feedstock were analysed, finding cysteine ranging from 1.56 to 2.26 mu g/mL and cystine from 1062.02 to 1348.13 mu g/mL (C) 2010 Elsevier B.V. All rights reserved.
AB - Two rapid, sensitive and quantitative methods for the determination of the cysteine and cystine ratio in complex defined media feedstock using monolithic reversed-phase liquid chromatography (RPLC) and RPLC-MS are presented. Cysteine is pre-derivatised with purified 2-chloro-1-methylquinolinium tetrafluoroborate (CMQT) and separated from other derivatisation products on a narrow-bore 50 mm x 2 mm I.D. monolithic C-18 column with UV detection at 355 nm. For reversed-phase LC (RPLC) the separation is carried out isocratically using a mobile phase of 50 mM trichloroacetic acid (TCA) adjusted to pH 2.5 with lithium hydroxide (LiOH) and acetonitrile (83:14) pumped at 1.5 mL/min with an elevated column temperature. For RPLC-MS an ammonium acetate and acetonitrile gradient method was developed with a reduced flow rate of 0.3 mL/min. The treatment of the samples consisted of dividing them into two aliquots, the first aliquot is analysed for cysteine and the second aliquot is analysed for cystine after its quantitative reduction to cysteine using tris(2-carboxyethyl)phosphine (TCEP). Both methods are linear, with R-2 > 0.999 for 0.25-500 mu M for cysteine and 0.25-250 mu M for cystine using the LC-UV method, sensitive, with detection limit of 36 nM for cysteine, and precise, with <= 1.1% RSD for both retention time and peak area (n = 6). Samples (n = 31) of an industry standard and supplied chemically defined media feedstock were analysed, finding cysteine ranging from 1.56 to 2.26 mu g/mL and cystine from 1062.02 to 1348.13 mu g/mL (C) 2010 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.chroma.2010.04.036
DO - 10.1016/j.chroma.2010.04.036
M3 - Article
VL - 1217
SP - 3863
EP - 3870
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 24
ER -