Effect of Astrocytic Energy Metabolism Depressant on 14C-Acetate Uptake in Intact Rat Brain

Fluorocitrate, a selective astrocytic toxin, was microinjected into the right striatum of rat brain, and the regional distribution of C-14-acetate was measured using autoradiography. A significant reduction (more than 80%) in C-14-acetate uptake over a 5-minute period was observed in the right striatum, compared with that in the left striatum (saline infused), 4 hours after fluorocitrate (1 nmol/muL) infusion. This effect was transient, and C-14-acetate uptake had almost returned to normal at 24 hours after the fluorocitrate infusion. In contrast, the regional blood flow in the striatum, as determined using C-14- iodoamphetarnine, was significantly increased by the fluorocitrate infusion. The present observations indicate that C-14- acetate uptake might be a useful characteristic for examining astrocytic energy metabolism in the intact brain.