TY - JOUR
T1 - Effective anti-tumor immunity induced in mice by a two-step vaccination protocol
AU - Martino, A
AU - Vismara, D
AU - Cicconi, R
AU - Delpino, A
AU - Ivanyi, J
AU - Colizzi, V
AU - Cassol, M
AU - Fraziano, M
AU - Piselli, P
PY - 2001
Y1 - 2001
N2 - Background: TS/A cells (a Balb/c-derived tumor cell line), when injected into syngenic mice, give rise to rapidly growing tumors. In this study, a vaccination protocol was established which was able to elicit an immune response effective in controlling tumor growth. Materials and Methods: T19.2.1, a TS/A clone enginereed to stably express the mycobacterial cell wall-associated 19-kDa lipoprotein, was used as cell vaccine to immunize Mycobacterium Bovis-BCG pre-immunized Balb/c mice. Results: Mice receiving the two-step vaccination protocol were able to develop a strong anti-TS/A DTH reaction. Moreover, following a challenge with wild-type TS/A cells, some vaccinated animals rejected the tumor and the remaining animals showed a significantly increased survival in respect to controls. Conclusion: The expression on TS/A cells of the mycobacterial 19-kDa antigen, recognised in the context of a pre-existing memory, immune response, promotes the immunological recognition of the otherwise non-immunogenic wild-type TS/A cells.
AB - Background: TS/A cells (a Balb/c-derived tumor cell line), when injected into syngenic mice, give rise to rapidly growing tumors. In this study, a vaccination protocol was established which was able to elicit an immune response effective in controlling tumor growth. Materials and Methods: T19.2.1, a TS/A clone enginereed to stably express the mycobacterial cell wall-associated 19-kDa lipoprotein, was used as cell vaccine to immunize Mycobacterium Bovis-BCG pre-immunized Balb/c mice. Results: Mice receiving the two-step vaccination protocol were able to develop a strong anti-TS/A DTH reaction. Moreover, following a challenge with wild-type TS/A cells, some vaccinated animals rejected the tumor and the remaining animals showed a significantly increased survival in respect to controls. Conclusion: The expression on TS/A cells of the mycobacterial 19-kDa antigen, recognised in the context of a pre-existing memory, immune response, promotes the immunological recognition of the otherwise non-immunogenic wild-type TS/A cells.
UR - http://www.scopus.com/inward/record.url?scp=0034768223&partnerID=8YFLogxK
M3 - Article
VL - 15
SP - 425
EP - 428
JO - In Vivo
JF - In Vivo
IS - 5
ER -
