Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells

Heba Badraiq; Aleksandra Cvoro; Antonio Galleu; Marisa Simon; Cristian Miere; Carl Hobbs; Reiner Schulz; Richard Siow; Francesco Dazzi; Dusko Ilic

doi:10.1038/s41598-017-18034-1

Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells

Heba Badraiq, Aleksandra Cvoro, Antonio Galleu, Marisa Simon, Cristian Miere, Carl Hobbs, Reiner Schulz, Richard Siow, Francesco Dazzi, Dusko Ilic^*

^*Corresponding author for this work

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Abstract

We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity. Genome-wide DNA methylation of triple positive (CD73⁺CD90⁺CD105⁺) WJ MSCs found 67 genes with at least one CpG site where the methylation difference was ≥0.2 in four or more obese donors. Only one gene, PNPLA7, demonstrated significant difference on methylome, transcriptome and protein level. Although the number of analysed donors is limited, our data suggest that the altered metabolic environment related to excessive body weight might bear consequences on the WJ MSCs.

Original language	English
Article number	17595
Journal	Scientific Reports
Volume	7
Issue number	1
Early online date	14 Dec 2017
DOIs	https://doi.org/10.1038/s41598-017-18034-1
Publication status	Published - Dec 2017

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AU - Badraiq, Heba

AU - Cvoro, Aleksandra

AU - Galleu, Antonio

AU - Simon, Marisa

AU - Miere, Cristian

AU - Hobbs, Carl

AU - Schulz, Reiner

AU - Siow, Richard

AU - Dazzi, Francesco

AU - Ilic, Dusko

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Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells

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