Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion

James R. Thompson; Andrew J. Heron; Yusdi Santoso; Mark I. Wallace

doi:10.1021/nl071943y

Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion

James R. Thompson, Andrew J. Heron, Yusdi Santoso, Mark I. Wallace

Chemistry

Research output: Contribution to journal › Article › peer-review

70 Citations (Scopus)

Abstract

We form artificial lipid bilayers suitable for single-molecule fluorescence microscopy by contacting an aqueous droplet with a hydrogel support immersed in a solution of lipid in oil. Our results show that droplet on hydrogel bilayers (DHBs) have high lipid mobilities, similar to those observed in unsupported lipid bilayers. DHBs are also stable over a period of several weeks. We examine membrane protein diffusion in these bilayers and report a decreased lateral mobility of the heptameric β-barrel pore-forming toxin α-hemolysin versus that of its monomeric precursor. These results corroborate previous models of the α-hemolysin insertion mechanism where the monomer binds to the lipid bilayer without insertion.

Original language	English
Pages (from-to)	3875-3878
Number of pages	4
Journal	Nano Letters
Volume	7
Issue number	12
DOIs	https://doi.org/10.1021/nl071943y
Publication status	Published - 1 Dec 2007

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title = "Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion",

abstract = "We form artificial lipid bilayers suitable for single-molecule fluorescence microscopy by contacting an aqueous droplet with a hydrogel support immersed in a solution of lipid in oil. Our results show that droplet on hydrogel bilayers (DHBs) have high lipid mobilities, similar to those observed in unsupported lipid bilayers. DHBs are also stable over a period of several weeks. We examine membrane protein diffusion in these bilayers and report a decreased lateral mobility of the heptameric β-barrel pore-forming toxin α-hemolysin versus that of its monomeric precursor. These results corroborate previous models of the α-hemolysin insertion mechanism where the monomer binds to the lipid bilayer without insertion.",

author = "Thompson, {James R.} and Heron, {Andrew J.} and Yusdi Santoso and Wallace, {Mark I.}",

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T1 - Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion

AU - Thompson, James R.

AU - Heron, Andrew J.

AU - Santoso, Yusdi

AU - Wallace, Mark I.

PY - 2007/12/1

Y1 - 2007/12/1

N2 - We form artificial lipid bilayers suitable for single-molecule fluorescence microscopy by contacting an aqueous droplet with a hydrogel support immersed in a solution of lipid in oil. Our results show that droplet on hydrogel bilayers (DHBs) have high lipid mobilities, similar to those observed in unsupported lipid bilayers. DHBs are also stable over a period of several weeks. We examine membrane protein diffusion in these bilayers and report a decreased lateral mobility of the heptameric β-barrel pore-forming toxin α-hemolysin versus that of its monomeric precursor. These results corroborate previous models of the α-hemolysin insertion mechanism where the monomer binds to the lipid bilayer without insertion.

AB - We form artificial lipid bilayers suitable for single-molecule fluorescence microscopy by contacting an aqueous droplet with a hydrogel support immersed in a solution of lipid in oil. Our results show that droplet on hydrogel bilayers (DHBs) have high lipid mobilities, similar to those observed in unsupported lipid bilayers. DHBs are also stable over a period of several weeks. We examine membrane protein diffusion in these bilayers and report a decreased lateral mobility of the heptameric β-barrel pore-forming toxin α-hemolysin versus that of its monomeric precursor. These results corroborate previous models of the α-hemolysin insertion mechanism where the monomer binds to the lipid bilayer without insertion.

U2 - 10.1021/nl071943y

DO - 10.1021/nl071943y

M3 - Article

SN - 1530-6984

VL - 7

SP - 3875

EP - 3878

JO - Nano Letters

JF - Nano Letters

IS - 12

ER -

Enhanced Stability and Fluidity in Droplet on Hydrogel Bilayers for Measuring Membrane Protein Diffusion

Abstract

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