Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

Niki Kourmouli; Yuh-Man Sun; Sjaak van der Sar; Prim B Singh; Jeremy P Brown

doi:10.1016/j.bbrc.2005.09.132

Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

Niki Kourmouli, Yuh-Man Sun, Sjaak van der Sar, Prim B Singh, Jeremy P Brown

King's College London

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only “deciphers” the histone code but can also “encode it.”

Original language	English
Pages (from-to)	901 - 907
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	337
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2005.09.132
Publication status	Published - 25 Nov 2005

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title = "Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1",

abstract = "We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only “deciphers” the histone code but can also “encode it.”",

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AU - Kourmouli, Niki

AU - Sun, Yuh-Man

AU - van der Sar, Sjaak

AU - Singh, Prim B

AU - Brown, Jeremy P

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AB - We developed a model system whereby HP1 can be targeted to pericentric heterochromatin in ES cells lacking Suv(3)9h1/2 histone methyltransferase (HMTase) activities. HP1 so targeted can reconstitute tri-methylated lysine 9 of histone H3 (Me(3)K9H3) and tri-methylated lysine 20 of histone H4 (Me(3)K20H4) at pericentric heterochromatin, indicating that HP1 can regulate the distribution of these histone modifications in vivo. Both homo- and hetero-typic interactions between the HP1 isotypes were demonstrated in vivo as were HP1 interactions with the ESET/SETDB1 HMTase and the ATRX chromatin remodelling enzyme. We conclude that HP1 not only “deciphers” the histone code but can also “encode it.”

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JF - Biochemical and Biophysical Research Communications

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Epigenetic regulation of mammalian pericentric heterochromatin in vivo by HP1

Abstract

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