TY - JOUR
T1 - Ferulic acid protects HepG2 cells and mouse liver from iron-induced damage
AU - Kose, Tugba
AU - Moreno-Fernandez, Jorge
AU - Vera-Aviles, Mayra
AU - Sharp, Paul A.
AU - Latunde-Dada, Gladys O.
N1 - Funding Information:
This research received no external funding. Tugba Kose received financial sponsorship and a scholarship from the Turkish Government Ministry of National Education.The authors declare the following financial interests/personal relationships which may be considered as potential competing interests:Tugba Kose reports administrative support and equipment, drugs, or supplies were provided by King's College London.
Funding Information:
This research received no external funding. Tugba Kose received financial sponsorship and a scholarship from the Turkish Government Ministry of National Education .
Publisher Copyright:
© 2023
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D.
AB - Liver as iron storage organ is particularly susceptible to oxidative stress-induced injury from excess iron. Thus, antioxidant therapies are often used to reverse oxidative damage and protect cells and tissues. This study investigated the protective effects of phenolic acids; ferulic acid (FA) and its metabolite, ferulic acid 4-O-sulfate disodium salt (FAS) against oxidative stress under iron overload conditions in mouse and HepG2 cells. Cells were exposed to FA or FAS and then treated with iron-induced oxidative stress complex of 50 μmol/L FAC and 20 μmol/L of 8-hydroxyquinoline 8HQ (8HQ-FAC). Iron dextran was injected intraperitoneally on alternate days for 10 days to induce the iron overload condition in BALB/c mice. The study revealed that the phenolic acids were protective against ROS production, lipid peroxidation and antioxidant depletion in HepG2 cells and liver tissues of BALB/c mice during iron-induced oxidative stress. The protective function of phenolic acids was achieved by the transcriptional activation of nuclear factor erythroid-2-related factor 2 (Nrf2) to regulate antioxidant genes. In conclusion, the study provides evidence that FA has the potential as a therapeutic agent against iron-related diseases such as T2D.
UR - http://www.scopus.com/inward/record.url?scp=85166223626&partnerID=8YFLogxK
U2 - 10.1016/j.bbrep.2023.101521
DO - 10.1016/j.bbrep.2023.101521
M3 - Article
SN - 2405-5808
VL - 35
JO - Biochemistry and Biophysics Reports
JF - Biochemistry and Biophysics Reports
M1 - 101521
ER -