Functional analysis of CD59 using complement-dependent cytotoxicity assay

Martin Kolev

doi:10.1007/978-1-62703-724-2_29

Functional analysis of CD59 using complement-dependent cytotoxicity assay

Martin Kolev^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

CD59 overexpression has been shown to confer the resistance of tumors to complement lysis. Complement lysis is one of the two major killing mechanisms of therapeutic anticancer antibodies. This chapter provides a method that allows studying the extent of complement protection of tumors by CD59.

Original language	English
Title of host publication	The Complement System: Methods and Protocols
Publisher	Humana Press Inc
Pages	347-353
Number of pages	7
Volume	1100
ISBN (Print)	9781627037235
DOIs	https://doi.org/10.1007/978-1-62703-724-2_29
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1100
ISSN (Print)	10643745

Keywords

Anti-disialoganglioside GD2 antibody
Cancer
Cell lines
Complement-dependent cytotoxicity
Fab fragments
Normal human serum

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-62703-724-2_29

Cite this

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title = "Functional analysis of CD59 using complement-dependent cytotoxicity assay",

abstract = "CD59 overexpression has been shown to confer the resistance of tumors to complement lysis. Complement lysis is one of the two major killing mechanisms of therapeutic anticancer antibodies. This chapter provides a method that allows studying the extent of complement protection of tumors by CD59.",

keywords = "Anti-disialoganglioside GD2 antibody, Cancer, Cell lines, Complement-dependent cytotoxicity, Fab fragments, Normal human serum",

author = "Martin Kolev",

year = "2014",

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isbn = "9781627037235",

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PY - 2014

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KW - Anti-disialoganglioside GD2 antibody

KW - Cancer

KW - Cell lines

KW - Complement-dependent cytotoxicity

KW - Fab fragments

KW - Normal human serum

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U2 - 10.1007/978-1-62703-724-2_29

DO - 10.1007/978-1-62703-724-2_29

M3 - Chapter

AN - SCOPUS:84934434544

SN - 9781627037235

VL - 1100

T3 - Methods in Molecular Biology

SP - 347

EP - 353

BT - The Complement System: Methods and Protocols

PB - Humana Press Inc

ER -

Functional analysis of CD59 using complement-dependent cytotoxicity assay

Abstract

Publication series

Keywords

UN SDGs

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