Functional modulation of human monocytes derived DCs by anaphylatoxins C3a and C5a

Ke Li; Henrieta Fazekasova; Naiyin Wang; Qi Peng; Steven H. Sacks; Giovanna Lombardi; Wuding Zhou

doi:10.1016/j.imbio.2011.07.033

Functional modulation of human monocytes derived DCs by anaphylatoxins C3a and C5a

Ke Li, Henrieta Fazekasova, Naiyin Wang, Qi Peng, Steven H. Sacks, Giovanna Lombardi, Wuding Zhou

Xi'an Jiaotong University

Research output: Contribution to journal › Article › peer-review

88 Citations (Scopus)

Abstract

Anaphylatoxins C3a and C5a are important modulators for dendritic cell activation and function in mice. In order to verify the significance of these observations in man, we have investigated the functional modulation of human monocytes derived DCs by C3a and C5a. Here we report that engagement of C3aR or C5aR on human monocytes derived DCs (moDCs) enhances the cell activation and their capacity for allostimulation. In addition, we show that intracellular production of cAMP is reduced and PI3K/AKT, ERK and NF-kappa B signalling is increased following stimulation with C3a or C5a, identifying intracellular signalling pathways that could convert cell surface C3aR and C5aR. engagement into changes in moDC functions. Our data provide evidence that human DCs are equipped to react to C3a/C5a and undergo phenotypic change as well as functional modulation. Complement offers a potential route to modulate human DC function and regulate T cell mediated immunity. (C) 2011 Elsevier GmbH. All rights reserved.

Original language	English
Pages (from-to)	65 - 73
Number of pages	9
Journal	Immunobiology
Volume	217
Issue number	1
DOIs	https://doi.org/10.1016/j.imbio.2011.07.033
Publication status	Published - Jan 2012

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title = "Functional modulation of human monocytes derived DCs by anaphylatoxins C3a and C5a",

abstract = "Anaphylatoxins C3a and C5a are important modulators for dendritic cell activation and function in mice. In order to verify the significance of these observations in man, we have investigated the functional modulation of human monocytes derived DCs by C3a and C5a. Here we report that engagement of C3aR or C5aR on human monocytes derived DCs (moDCs) enhances the cell activation and their capacity for allostimulation. In addition, we show that intracellular production of cAMP is reduced and PI3K/AKT, ERK and NF-kappa B signalling is increased following stimulation with C3a or C5a, identifying intracellular signalling pathways that could convert cell surface C3aR and C5aR. engagement into changes in moDC functions. Our data provide evidence that human DCs are equipped to react to C3a/C5a and undergo phenotypic change as well as functional modulation. Complement offers a potential route to modulate human DC function and regulate T cell mediated immunity. (C) 2011 Elsevier GmbH. All rights reserved.",

author = "Ke Li and Henrieta Fazekasova and Naiyin Wang and Qi Peng and Sacks, {Steven H.} and Giovanna Lombardi and Wuding Zhou",

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T1 - Functional modulation of human monocytes derived DCs by anaphylatoxins C3a and C5a

AU - Li, Ke

AU - Fazekasova, Henrieta

AU - Wang, Naiyin

AU - Peng, Qi

AU - Sacks, Steven H.

AU - Lombardi, Giovanna

AU - Zhou, Wuding

PY - 2012/1

Y1 - 2012/1

N2 - Anaphylatoxins C3a and C5a are important modulators for dendritic cell activation and function in mice. In order to verify the significance of these observations in man, we have investigated the functional modulation of human monocytes derived DCs by C3a and C5a. Here we report that engagement of C3aR or C5aR on human monocytes derived DCs (moDCs) enhances the cell activation and their capacity for allostimulation. In addition, we show that intracellular production of cAMP is reduced and PI3K/AKT, ERK and NF-kappa B signalling is increased following stimulation with C3a or C5a, identifying intracellular signalling pathways that could convert cell surface C3aR and C5aR. engagement into changes in moDC functions. Our data provide evidence that human DCs are equipped to react to C3a/C5a and undergo phenotypic change as well as functional modulation. Complement offers a potential route to modulate human DC function and regulate T cell mediated immunity. (C) 2011 Elsevier GmbH. All rights reserved.

AB - Anaphylatoxins C3a and C5a are important modulators for dendritic cell activation and function in mice. In order to verify the significance of these observations in man, we have investigated the functional modulation of human monocytes derived DCs by C3a and C5a. Here we report that engagement of C3aR or C5aR on human monocytes derived DCs (moDCs) enhances the cell activation and their capacity for allostimulation. In addition, we show that intracellular production of cAMP is reduced and PI3K/AKT, ERK and NF-kappa B signalling is increased following stimulation with C3a or C5a, identifying intracellular signalling pathways that could convert cell surface C3aR and C5aR. engagement into changes in moDC functions. Our data provide evidence that human DCs are equipped to react to C3a/C5a and undergo phenotypic change as well as functional modulation. Complement offers a potential route to modulate human DC function and regulate T cell mediated immunity. (C) 2011 Elsevier GmbH. All rights reserved.

U2 - 10.1016/j.imbio.2011.07.033

DO - 10.1016/j.imbio.2011.07.033

M3 - Article

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SP - 65

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JO - Immunobiology

JF - Immunobiology

IS - 1

ER -

Functional modulation of human monocytes derived DCs by anaphylatoxins C3a and C5a

Abstract

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