Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Laureen Jacquet; Heema Hewitson; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Carl Hobbs; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2016.01.012

Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene

Laureen Jacquet, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

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Abstract

The KCL012 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (46 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

Original language	English
Pages (from-to)	264-267
Number of pages	4
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	14 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2016.01.012
Publication status	Published - Mar 2016

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Generation of KCL012 research_JACQUET_Publishedonline14January2016_GOLD VoR (CC BY)Final published version, 1.26 MBLicence: CC BY

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AU - Jacquet, Laureen

AU - Hewitson, Heema

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Hobbs, Carl

AU - Stephenson, Emma

AU - Ilic, Dusko

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AB - The KCL012 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (46 trinucleotide repeats; 17 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

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Generation of KCL012 research grade human embryonic stem cell line carrying a mutation in the HTT gene

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