Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

Cristian Miere; Heema Hewitson; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.003

Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

Cristian Miere, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

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Abstract

The KCL016 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting splicing site of the VHL gene encoding von Hippel-Lindau tumor suppressor E3 ubiquitin protein ligase (676 + 3A > T). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Original language	English
Pages (from-to)	37-39
Number of pages	3
Journal	Stem Cell Research
Volume	16
Issue number	1
Early online date	3 Dec 2015
DOIs	https://doi.org/10.1016/j.scr.2015.12.003
Publication status	Published - Jan 2016

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Generation of KCL016 research_MIERE_Publishedonline3December2016_GOLD VoR (CC BY-NC-ND)Final published version, 531 KBLicence: CC BY-NC-ND

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AU - Miere, Cristian

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AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

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Generation of KCL016 research grade human embryonic stem cell line carrying a mutation in VHL gene

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