Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

Cristian Miere; Heema Hewitson; Liani Devito; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2016.01.004

Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

Cristian Miere, Heema Hewitson, Liani Devito, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

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Abstract

The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Original language	English
Pages (from-to)	342-344
Number of pages	3
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	15 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2016.01.004
Publication status	Published - Mar 2016

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Generation of KCL018 research_MIERE_Publishedonline15January2016_GOLD VoR (CC BY)Final published version, 761 KBLicence: CC BY

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abstract = "The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.",

author = "Cristian Miere and Heema Hewitson and Liani Devito and Victoria Wood and Neli Kadeva and Glenda Cornwell and Stefano Codognotto and Emma Stephenson and Dusko Ilic",

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AU - Miere, Cristian

AU - Hewitson, Heema

AU - Devito, Liani

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

PY - 2016/3

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N2 - The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

AB - The KCL018 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the DMPK gene encoding the dystrophia myotonica protein kinase (2200 trinucleotide repeats; 14 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

U2 - 10.1016/j.scr.2016.01.004

DO - 10.1016/j.scr.2016.01.004

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SP - 342

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JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Generation of KCL018 research grade human embryonic stem cell line carrying a mutation in the DMPK gene

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