Generation of KCL021 research grade human embryonic stem cell line carrying a δF508 mutation in the CFTR gene

Cristian Miere; Heema Hewitson; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.042

Generation of KCL021 research grade human embryonic stem cell line carrying a δF508 mutation in the CFTR gene

Cristian Miere^*, Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic

^*Corresponding author for this work

Women & Children's Health

King's College London

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Abstract

The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a δF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Original language	English
Pages (from-to)	177-179
Number of pages	3
Journal	Stem Cell Research
Volume	16
Issue number	1
Early online date	6 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.042
Publication status	Published - Jan 2016

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Generation of KCL021 research_MIERE_Publishedonline6January2016_GOLD VoR (CC BY)Final published version, 586 KBLicence: CC BY

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abstract = "The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a δF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.",

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AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

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AB - The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a δF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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Generation of KCL021 research grade human embryonic stem cell line carrying a δF508 mutation in the CFTR gene

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