Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

Heema Hewitson; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2016.01.009

Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

Heema Hewitson, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

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Abstract

The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Original language	English
Pages (from-to)	256-258
Number of pages	3
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	14 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2016.01.009
Publication status	Published - Mar 2016

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Generation of KCL025 research_HEWITSON_Publishedonline14January2016_GOLD VoR (CC BY)Final published version, 643 KBLicence: CC BY

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title = "Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene",

abstract = "The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.",

author = "Heema Hewitson and Victoria Wood and Neli Kadeva and Glenda Cornwell and Stefano Codognotto and Emma Stephenson and Dusko Ilic",

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T1 - Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

AU - Hewitson, Heema

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

PY - 2016/3

Y1 - 2016/3

N2 - The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

AB - The KCL025 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation in the NF1 gene encoding neurofibromin (c.3739-3742 ΔTTTG). Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.

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SN - 1873-5061

VL - 16

SP - 256

EP - 258

JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Generation of KCL025 research grade human embryonic stem cell line carrying a mutation in NF1 gene

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