Generation of KCL031 clinical grade human embryonic stem cell line

Laureen Jacquet; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Carl Hobbs; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.033

Generation of KCL031 clinical grade human embryonic stem cell line

Laureen Jacquet, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Carl Hobbs, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

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Abstract

The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

Original language	English
Pages (from-to)	195-198
Number of pages	4
Journal	Stem Cell Research
Volume	16
Issue number	1
Early online date	3 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.033
Publication status	Published - Jan 2016

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Generation of KCL031 clinical_JACQUET_Publishedonline3January2016_GOLD VoR (CC BY)Final published version, 1.23 MBLicence: CC BY

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AU - Jacquet, Laureen

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Hobbs, Carl

AU - Stephenson, Emma

AU - Ilic, Dusko

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AB - The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.

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Generation of KCL031 clinical grade human embryonic stem cell line

Abstract

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