Generation of KCL033 clinical grade human embryonic stem cell line

Liani Devito; Anastasia Petrova; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.047

Generation of KCL033 clinical grade human embryonic stem cell line

Liani Devito, Anastasia Petrova, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

110 Downloads (Pure)

Abstract

The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.

Original language	English
Pages (from-to)	296-299
Number of pages	4
Journal	Stem Cell Research
Volume	16
Issue number	2
Early online date	15 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.047
Publication status	Published - Mar 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.scr.2015.12.047Licence: CC BY

Generation of KCL033 clinical_DEVITO_Publishedonline15January2016_GOLD VoR (CC BY)Final published version, 495 KBLicence: CC BY

Cite this

@article{7d18f27652234a03b46cd8acb5253489,

title = "Generation of KCL033 clinical grade human embryonic stem cell line",

abstract = "The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.",

author = "Liani Devito and Anastasia Petrova and Victoria Wood and Neli Kadeva and Glenda Cornwell and Stefano Codognotto and Emma Stephenson and Dusko Ilic",

year = "2016",

month = mar,

doi = "10.1016/j.scr.2015.12.047",

language = "English",

volume = "16",

pages = "296--299",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Generation of KCL033 clinical grade human embryonic stem cell line

AU - Devito, Liani

AU - Petrova, Anastasia

AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

PY - 2016/3

Y1 - 2016/3

N2 - The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.

AB - The KCL033 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility and specific and non-specific human pathogens.

UR - http://www.scopus.com/inward/record.url?scp=84955596988&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2015.12.047

DO - 10.1016/j.scr.2015.12.047

M3 - Article

C2 - 27345988

AN - SCOPUS:84955596988

SN - 1873-5061

VL - 16

SP - 296

EP - 299

JO - Stem Cell Research

JF - Stem Cell Research

IS - 2

ER -

Generation of KCL033 clinical grade human embryonic stem cell line

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this