Generation of KCL039 clinical grade human embryonic stem cell line

Liani Devito; Victoria Wood; Neli Kadeva; Glenda Cornwell; Stefano Codognotto; Emma Stephenson; Dusko Ilic

doi:10.1016/j.scr.2015.12.036

Generation of KCL039 clinical grade human embryonic stem cell line

Liani Devito, Victoria Wood, Neli Kadeva, Glenda Cornwell, Stefano Codognotto, Emma Stephenson, Dusko Ilic^*

^*Corresponding author for this work

Women & Children's Health

King's College London

Research output: Contribution to journal › Article › peer-review

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Abstract

The KCL039 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.

Original language	English
Pages (from-to)	170-172
Number of pages	3
Journal	Stem Cell Research
Volume	16
Issue number	1
Early online date	3 Jan 2016
DOIs	https://doi.org/10.1016/j.scr.2015.12.036
Publication status	Published - Jan 2016

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Generation of KCL039 clinical_DEVITO_Publishedonline3January2016_GOLD VoR (CC BY)Final published version, 642 KBLicence: CC BY

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abstract = "The KCL039 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.",

author = "Liani Devito and Victoria Wood and Neli Kadeva and Glenda Cornwell and Stefano Codognotto and Emma Stephenson and Dusko Ilic",

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AU - Wood, Victoria

AU - Kadeva, Neli

AU - Cornwell, Glenda

AU - Codognotto, Stefano

AU - Stephenson, Emma

AU - Ilic, Dusko

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Generation of KCL039 clinical grade human embryonic stem cell line

Abstract

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