TY - JOUR
T1 - High rates of clustering of strains causing tuberculosis in harare, Zimbabwe: A molecular epidemiological study
AU - Easterbrook, P J
AU - Gibson, A
AU - Murad, S
AU - Lamprecht, D
AU - Ives, N
AU - Ferguson, A
AU - Lowe, O
AU - Mason, P
AU - Ndudzo, A
AU - Taziwa, A
AU - Makombe, R
AU - Mbengeranwa, L
AU - Sola, C
AU - Rostogi, N
AU - Drobniewski, F
PY - 2004/10
Y1 - 2004/10
N2 - We examined the pattern of tuberculosis (TB) transmission (i.e., reactivation versus recent transmission) and the impact of human immunodeficiency virus (HIV) infection in Harare, Zimbabwe. Consecutive adult smear-positive pulmonary TB patients presenting to an urban hospital in Harare were enrolled. A detailed epidemiological questionnaire was completed, and tests for HIV type 1 and CD4 cell counts were performed for each patient. Molecular fingerprinting of the genomic DNA recovered from cultures of sputum was performed by two molecular typing methods: spacer oligonucleotide typing (spoligotyping) and analysis of variable number of tandem DNA repeats (VNTRs). A cluster was defined as isolates from two or more patients that shared the same spoligotype pattern or the same VNTR pattern, or both. DNA suitable for typing was recovered from 224 patients. The prevalence of HIV infection was 79%. Of 187 patient isolates (78.6%) typed by both spoligotyping and analysis of VNTRs, 147 were identified as part of a cluster by both methods. By spoligotyping alone, 84.1% of patient isolates were grouped into 20 clusters. The cluster size was generally
AB - We examined the pattern of tuberculosis (TB) transmission (i.e., reactivation versus recent transmission) and the impact of human immunodeficiency virus (HIV) infection in Harare, Zimbabwe. Consecutive adult smear-positive pulmonary TB patients presenting to an urban hospital in Harare were enrolled. A detailed epidemiological questionnaire was completed, and tests for HIV type 1 and CD4 cell counts were performed for each patient. Molecular fingerprinting of the genomic DNA recovered from cultures of sputum was performed by two molecular typing methods: spacer oligonucleotide typing (spoligotyping) and analysis of variable number of tandem DNA repeats (VNTRs). A cluster was defined as isolates from two or more patients that shared the same spoligotype pattern or the same VNTR pattern, or both. DNA suitable for typing was recovered from 224 patients. The prevalence of HIV infection was 79%. Of 187 patient isolates (78.6%) typed by both spoligotyping and analysis of VNTRs, 147 were identified as part of a cluster by both methods. By spoligotyping alone, 84.1% of patient isolates were grouped into 20 clusters. The cluster size was generally
UR - http://www.scopus.com/inward/record.url?scp=5444248384&partnerID=8YFLogxK
U2 - 10.1128/JCM.42.10.4536-4544.2004
DO - 10.1128/JCM.42.10.4536-4544.2004
M3 - Article
VL - 42
SP - 4536
EP - 4544
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 10
ER -
