Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

Priyank Shah; Brad Littleton; Thomas Kavanagh; Yu Nie; Stephen Sturzenbaum; David Richards

doi:10.1117/12.2510052

Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

Priyank Shah, Brad Littleton, Thomas Kavanagh, Yu Nie, Stephen Sturzenbaum, David Richards

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

Abstract

Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIX
Editors	Ammasi Periasamy, Peter T. C. So, Karsten Konig
Publisher	SPIE
Volume	10882
ISBN (Electronic)	9781510624061
DOIs	https://doi.org/10.1117/12.2510052
Publication status	Published - 22 Feb 2019
Event	Multiphoton Microscopy in the Biomedical Sciences XIX 2019 - San Francisco, United States Duration: 3 Feb 2019 → 6 Feb 2019

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIX 2019
Country/Territory	United States
City	San Francisco
Period	3/02/2019 → 6/02/2019

Keywords

Coherent anti-Stokes Raman scattering
Non-resonant background
Polarized Raman
Raman depolarization

Access to Document

10.1117/12.2510052

Cite this

@inbook{3a474e1e3c1e445e8d6261b161a7e317,

title = "Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics",

abstract = "Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.",

keywords = "Coherent anti-Stokes Raman scattering, Non-resonant background, Polarized Raman, Raman depolarization",

author = "Priyank Shah and Brad Littleton and Thomas Kavanagh and Yu Nie and Stephen Sturzenbaum and David Richards",

year = "2019",

month = feb,

day = "22",

doi = "10.1117/12.2510052",

language = "English",

volume = "10882",

editor = "Ammasi Periasamy and So, {Peter T. C.} and Karsten Konig",

booktitle = "Multiphoton Microscopy in the Biomedical Sciences XIX",

publisher = "SPIE",

address = "United States",

note = "Multiphoton Microscopy in the Biomedical Sciences XIX 2019 ; Conference date: 03-02-2019 Through 06-02-2019",

}

Shah, P, Littleton, B, Kavanagh, T , Nie, Y , Sturzenbaum, S & Richards, D 2019, Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics. in A Periasamy, PTC So & K Konig (eds), Multiphoton Microscopy in the Biomedical Sciences XIX. vol. 10882, 108822C, SPIE, Multiphoton Microscopy in the Biomedical Sciences XIX 2019, San Francisco, United States, 3/02/2019. https://doi.org/10.1117/12.2510052

Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics. / Shah, Priyank; Littleton, Brad; Kavanagh, Thomas et al.
Multiphoton Microscopy in the Biomedical Sciences XIX. ed. / Ammasi Periasamy; Peter T. C. So; Karsten Konig. Vol. 10882 SPIE, 2019. 108822C.

Research output: Chapter in Book/Report/Conference proceeding › Other chapter contribution › peer-review

TY - CHAP

T1 - Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

AU - Shah, Priyank

AU - Littleton, Brad

AU - Kavanagh, Thomas

AU - Nie, Yu

AU - Sturzenbaum, Stephen

AU - Richards, David

PY - 2019/2/22

Y1 - 2019/2/22

N2 - Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.

AB - Broadband coherent anti-Stokes Raman scattering (B-CARS) in a hybrid 2-color/3-color excitation regime has been shown to be a photon-efficient method of generating CARS across the entire vibrational region of interest (fingerprint and C-H stretch). We extend here our spectral interferometric polarized CARS (SIP-CARS) approach to a hybrid 2-color/3-color excitation regime, demonstrate that the power/polarization dependence for both 2- color and 3-color excitation agrees with theory, explore the application of SIP-CARS for the special case of anisotropic, birefringent materials (using collagen) and present in vivo lipid imaging of different mutations of the nematode Caenorhabditis elegans with modified lipid distributions. The extension of SIP-CARS to a hybrid excitation scheme makes possible a fast, all-optical and quantitative method for Raman imaging.

KW - Coherent anti-Stokes Raman scattering

KW - Non-resonant background

KW - Polarized Raman

KW - Raman depolarization

UR - http://www.scopus.com/inward/record.url?scp=85067824433&partnerID=8YFLogxK

U2 - 10.1117/12.2510052

DO - 10.1117/12.2510052

M3 - Other chapter contribution

AN - SCOPUS:85067824433

VL - 10882

BT - Multiphoton Microscopy in the Biomedical Sciences XIX

A2 - Periasamy, Ammasi

A2 - So, Peter T. C.

A2 - Konig, Karsten

PB - SPIE

T2 - Multiphoton Microscopy in the Biomedical Sciences XIX 2019

Y2 - 3 February 2019 through 6 February 2019

ER -

Hybrid 2-color/3-color background-free broadband CARS with passive polarization optics

Abstract

Conference

Keywords

Access to Document

Other files and links

Fingerprint

Cite this