@article{451bb9fea6c34fbe90e33a0ed8b533c0,
title = "In vitro elucidation of the crucial but complex oxidative tailoring steps in rufomycin biosynthesis enables one pot conversion of rufomycin B to rufomycin C",
abstract = "The antimycobacterial peptides, rufomycins, have their antibiotic activity conferred by oxidative tailoring of the cyclic peptide. Here we elucidate the roles of cytochrome P450s RufS and RufM in regioselective epoxidation and alkyl oxidation respectively and demonstrate how RufM and RufS create a complex product profile dependent on redox partner availability. Finally, we report the in vitro one pot conversion of rufomycin B to rufomycin C.",
author = "{Perez Ortiz}, Gustavo and John Sidda and {de los Santos}, Emmanuel and Hubert, {Catherine B.} and Barry, {Sarah M.}",
note = "Funding Information: We thank the MRC (MC_PC14105 v.2) & BBSRC (BB/ P019811/1) for grants to SMB, the Mexican National Council of Science & Technology (CONACyT)/Mexican Secretary of Energy (SENER) & KCL for scholarships to GPO & CBH respectively. ES was supported by the BBSRC & EPSRC (BB/ M017982/1). We thank the KCL Centre for Biomolecular Spectroscopy (Wellcome Trust, 202767/Z/16/Z) for high field NMR. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",
year = "2021",
month = nov,
day = "18",
doi = "10.1039/D1CC04794A",
language = "English",
volume = "57",
pages = "11795--11798",
journal = "Chem. Commun.",
publisher = "Royal Society of Chemistry",
number = "89",
}