In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset; Conor C Horgan; James P K Armstrong; Mads S Bergholt; Vincenzo Torraca; Qu Chen; Timothy J Keane; Laurence Bugeon; Margaret J Dallman; Serge Mostowy; Molly M Stevens

doi:10.1038/s41467-020-19827-1

In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Håkon Høgset, Conor C Horgan, James P K Armstrong, Mads S Bergholt, Vincenzo Torraca, Qu Chen, Timothy J Keane, Laurence Bugeon, Margaret J Dallman, Serge Mostowy, Molly M Stevens

Centre for Craniofacial & Regenerative Biology

Department of Materials, Imperial College London, London SW7 2AZ, United Kingdom; Department of Bioengineering, Imperial College London, London SW7 2AZ, United Kingdom; Institute of Biomedical Engineering, Imperial College London, London SW7 2AZ, United Kingdom.
Department of Craniofacial Development and Stem Cell Biology

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.

Original language	English
Article number	6172
Pages (from-to)	6172
Journal	Nature Communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-19827-1
Publication status	Published - 2 Dec 2020

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AU - Armstrong, James P K

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AU - Torraca, Vincenzo

AU - Chen, Qu

AU - Keane, Timothy J

AU - Bugeon, Laurence

AU - Dallman, Margaret J

AU - Mostowy, Serge

AU - Stevens, Molly M

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AB - Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos.

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In vivo biomolecular imaging of zebrafish embryos using confocal Raman spectroscopy

Abstract

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