TY - JOUR
T1 - Intranasal IFN gamma extends passive IgA antibody protection of mice against Mycobacterium tuberculosis lung infection
AU - Reljic, R
AU - Clark, S O
AU - Williams, A
AU - Falero-Diaz, G
AU - Singh, M
AU - Challacombe, S
AU - Marsh, P D
AU - Ivanyi, J
PY - 2006/3
Y1 - 2006/3
N2 - Intranasal inoculation of mice with monoclonal IgA against the alpha-crystallin (acr1) antigen can diminish the tuberculous infection in the lungs. As this effect has been observed only over a short-term, we investigated if it could be extended by inoculation of IFN gamma 3 days before infection, and further coinoculations with IgA, at 2 h before and 2 and 7 days after aerosol infection with Mycobacterium tuberculosis H37Rv. This treatment reduced the lung infection at 4 weeks more than either IgA or IFN gamma alone (i.e. 17-fold, from 4.2 x 10(7) to 2.5 x 10(6) CFU, P = 0.006), accompanied also by lower granulomatous infiltration of the lungs. IFN gamma added prior to infection of mouse peritoneal macrophages with IgA-opsonized bacilli resulted in a synergistic increase of nitric oxide and TNF alpha production and a 2-3 fold decrease in bacterial counts. Our improved results suggest, that combined treatment with IFN gamma and IgA could be developed towards prophylactic treatment of AIDS patients, or as an adjunct to chemotherapy
AB - Intranasal inoculation of mice with monoclonal IgA against the alpha-crystallin (acr1) antigen can diminish the tuberculous infection in the lungs. As this effect has been observed only over a short-term, we investigated if it could be extended by inoculation of IFN gamma 3 days before infection, and further coinoculations with IgA, at 2 h before and 2 and 7 days after aerosol infection with Mycobacterium tuberculosis H37Rv. This treatment reduced the lung infection at 4 weeks more than either IgA or IFN gamma alone (i.e. 17-fold, from 4.2 x 10(7) to 2.5 x 10(6) CFU, P = 0.006), accompanied also by lower granulomatous infiltration of the lungs. IFN gamma added prior to infection of mouse peritoneal macrophages with IgA-opsonized bacilli resulted in a synergistic increase of nitric oxide and TNF alpha production and a 2-3 fold decrease in bacterial counts. Our improved results suggest, that combined treatment with IFN gamma and IgA could be developed towards prophylactic treatment of AIDS patients, or as an adjunct to chemotherapy
U2 - 10.1111/j.1365-2249.2006.03012.x
DO - 10.1111/j.1365-2249.2006.03012.x
M3 - Article
SN - 1365-2249
VL - 143
SP - 467
EP - 473
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 3
ER -
