@inbook{71c2719b86844718b38cafdea03b265a,
title = "Isolation and Functional Characterization of Regulatory CD4+ T Cells from the Inflamed Joints of Patients with Rheumatoid Arthritis",
abstract = "Regulatory T cells play a critical role in maintaining immune homeostasis and in preventing and controlling unwanted immune activation. These cells are often studied in the context of human peripheral blood, but can also be isolated from other biofluids. Here we describe methods for the isolation and functional characterization of human CD4+ CD25hi CD127low regulatory T cells from the synovial fluid of patients with inflammatory arthritis.",
keywords = "Cell enrichment, Cell sorting, Joint, Monocyte modulation, Rheumatoid arthritis, Suppression assay, Synovial fluid, Tregs",
author = "Povoleri, {Giovanni A.M.} and Veerle Fleskens and Taams, {Leonie S.}",
note = "Funding Information: The authors acknowledge research funding support from Versus Arthritis (ref 21139), the IMI JU funded project BTCure (115142-2), and the Department of Health via the National Institute for Health Research (NIHR) comprehensive Biomedical Research Centre (BRC) award to Guy{\textquoteright}s & St. Thomas{\textquoteright} NHS Foundation Trust in partnership with King{\textquoteright}s College London and King{\textquoteright}s College Hospital NHS Foundation Trust. The views expressed are those of the author(s) and not necessarily those of the NHS, the NIHR, or the Department of Health. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2647-4_13",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc",
pages = "189--203",
booktitle = "Methods in Molecular Biology",
}