TY - JOUR
T1 - Isolation of a new iso-quinoline alkaloid and cytotoxicity studies of pure compounds and crude extracts of Ravenia spectabilis engl
AU - Tabassum, Fatema
AU - Islam, Sheikh Nazrul
AU - Tuz-Zohora, Fatema
AU - Hasan, Choudhury Mahmood
AU - Rahman, Khondaker Miraz
AU - Ahsan, Monira
N1 - Publisher Copyright:
© 2024 The Authors
PY - 2024/7/30
Y1 - 2024/7/30
N2 - A new 2-quinolone alkaloid, iso-oligophyline (1), and two very unusual C34 terpenoids, proposed names ravespanol (2) and ravespanone (3), along with two known compounds, β-sitosterol (4), and methyl linoleate (5), were isolated from the leaf extract of Ravenia spectabilis engl. Methyl linoleate constitutes the first report of isolation from this species. We have already reported the isolation of atanine (6), oligophyline (7), ravenoline (8), and arborinine (9) from the plant. Based on nuclear magnetic resonance (NMR) spectroscopy and mass spectrometric analysis, the structure of the isolated chemicals was determined. The crude fractions and four compounds (6,7,8 and 9) were evaluated for a cytotoxicity study on a panel of six human stomach cancer cell lines (SCL, SCL-6, SCL-37′6, SCL-9, K-3, N21) by MTT assay. Among the plant extracts and isolated compounds, petroleum ether fraction and compound 7 exhibited the highest cytotoxic activity against SCL and SCL-6 cells, where the IC50 values were 17.9 and 16.56 μM, respectively.
AB - A new 2-quinolone alkaloid, iso-oligophyline (1), and two very unusual C34 terpenoids, proposed names ravespanol (2) and ravespanone (3), along with two known compounds, β-sitosterol (4), and methyl linoleate (5), were isolated from the leaf extract of Ravenia spectabilis engl. Methyl linoleate constitutes the first report of isolation from this species. We have already reported the isolation of atanine (6), oligophyline (7), ravenoline (8), and arborinine (9) from the plant. Based on nuclear magnetic resonance (NMR) spectroscopy and mass spectrometric analysis, the structure of the isolated chemicals was determined. The crude fractions and four compounds (6,7,8 and 9) were evaluated for a cytotoxicity study on a panel of six human stomach cancer cell lines (SCL, SCL-6, SCL-37′6, SCL-9, K-3, N21) by MTT assay. Among the plant extracts and isolated compounds, petroleum ether fraction and compound 7 exhibited the highest cytotoxic activity against SCL and SCL-6 cells, where the IC50 values were 17.9 and 16.56 μM, respectively.
KW - 2-Quinolone alkaloid
KW - Cytotoxicity
KW - iso-oligophyline
KW - Ravenia spectabilis engl.
KW - Stomach cancer cell line
KW - Triterpene
UR - http://www.scopus.com/inward/record.url?scp=85198512330&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2024.e34508
DO - 10.1016/j.heliyon.2024.e34508
M3 - Article
AN - SCOPUS:85198512330
SN - 2405-8440
VL - 10
JO - Heliyon
JF - Heliyon
IS - 14
M1 - e34508
ER -
