TY - JOUR
T1 - Kinetics and distribution of alcohol oxidising activity in Acholeplasma and Mycoplasma species
AU - Abu-Amero, K K
AU - Abu-Groun, E A M
AU - Halablab, M A
AU - Miles, R J
PY - 2000/2/1
Y1 - 2000/2/1
N2 - Alcohol metabolism by Acholeplasma and Mycoplasma cell suspensions was determined using changes in dissolved oxygen tension to monitor oxygen uptake. All seven Acholeplasma test species oxidised ethanol and (where tested) propanol, butanol and pentanol. The rate of oxidation. at any particular substrate concentration, decreased with increasing alcohol molecular mass. Amongst 20 Mycoplasma species tested. M. agalactiae, M bovis, M. dispar, M. gallisepticum, M. pneumoniae and M. ovipneumoniae oxidised ethanol. Propanol was also oxidised by M. dispar and isopropanol by M. agalactiae, M. bovis and M. ovipneumoniae. Isopropanol was oxidised at particularly high rates (V-max > 100 nmol O-2 taken up min(-1) mg cell protein(-1)) and with a relatively high affinity (K-m value <2 mM); oxygen uptake was consistent with oxidation to acetone. The significance of alcohol oxidation is unclear, as it would not be predicted to lead to ATP synthesis. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
AB - Alcohol metabolism by Acholeplasma and Mycoplasma cell suspensions was determined using changes in dissolved oxygen tension to monitor oxygen uptake. All seven Acholeplasma test species oxidised ethanol and (where tested) propanol, butanol and pentanol. The rate of oxidation. at any particular substrate concentration, decreased with increasing alcohol molecular mass. Amongst 20 Mycoplasma species tested. M. agalactiae, M bovis, M. dispar, M. gallisepticum, M. pneumoniae and M. ovipneumoniae oxidised ethanol. Propanol was also oxidised by M. dispar and isopropanol by M. agalactiae, M. bovis and M. ovipneumoniae. Isopropanol was oxidised at particularly high rates (V-max > 100 nmol O-2 taken up min(-1) mg cell protein(-1)) and with a relatively high affinity (K-m value <2 mM); oxygen uptake was consistent with oxidation to acetone. The significance of alcohol oxidation is unclear, as it would not be predicted to lead to ATP synthesis. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=0033955536&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2000.tb08949.x
DO - 10.1111/j.1574-6968.2000.tb08949.x
M3 - Article
VL - 183
SP - 147
EP - 151
JO - Fems Microbiology Letters
JF - Fems Microbiology Letters
IS - 1
ER -