TY - JOUR
T1 - Massive parallel sequencing in forensics
T2 - advantages, issues, technicalities, and prospects
AU - Ballard, David
AU - Winkler-Galicki, Jakub
AU - Wesoły, Joanna
PY - 2020/7/1
Y1 - 2020/7/1
N2 - In the last decade, next-generation sequencing (NGS) technology, alternatively massive parallel sequencing (MPS), was applied to all fields of biological research. Its introduction to the field of forensics was slower, mainly due to lack of accredited sequencers, kits, and relatively higher sequencing error rates as compared with standardized Sanger sequencing. Currently, a majority of the problematic issues have been solved, which is proven by the body of reports in the literature. Here, we discuss the utility of NGS sequencing in forensics, emphasizing the advantages, issues, the technical aspects of the experiments, commercial solutions, and the potentially interesting applications of MPS.
AB - In the last decade, next-generation sequencing (NGS) technology, alternatively massive parallel sequencing (MPS), was applied to all fields of biological research. Its introduction to the field of forensics was slower, mainly due to lack of accredited sequencers, kits, and relatively higher sequencing error rates as compared with standardized Sanger sequencing. Currently, a majority of the problematic issues have been solved, which is proven by the body of reports in the literature. Here, we discuss the utility of NGS sequencing in forensics, emphasizing the advantages, issues, the technical aspects of the experiments, commercial solutions, and the potentially interesting applications of MPS.
KW - MPS
KW - Nanopore sequencing
KW - NGS
KW - Nucleic acids
KW - Sequencing by synthesis
UR - http://www.scopus.com/inward/record.url?scp=85086278751&partnerID=8YFLogxK
U2 - 10.1007/s00414-020-02294-0
DO - 10.1007/s00414-020-02294-0
M3 - Review article
C2 - 32451905
AN - SCOPUS:85086278751
SN - 0937-9827
VL - 134
SP - 1291
EP - 1303
JO - International Journal of Legal Medicine
JF - International Journal of Legal Medicine
IS - 4
ER -