Abstract
The complex nature of biofluids demands efficient, sensitive and high-resolution analytical methodologies to examine how the 'metabolic fingerprint' changes during disease. This paper describes how sulphated beta-cyclodextrin-modified micellar electrokinetic capillary chromatography (SbetaCD-MECC) has been combined with data alignment analysis and may prove a useful new tool in urine profiling, allowing for separation of over 80 urinary analytes in under 25 min. The optimised and validated SbetaCD-MECC methodology combined with data alignment analysis provides rapid identification of 'mismatches' between urine profiles which are not easily detected with the naked eye as well as a 'similarity score' which indicates the total sum of differences between one profile and another. The combination of SbetaCD-MECC with data alignment software should prove a useful alternative tool in metabonomic studies for rapid comparison of urine profiles. (C) 2003 Elsevier B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 203 - 212 |
| Number of pages | 10 |
| Journal | Journal of Chromatography A |
| Volume | 1027 |
| Issue number | 1-2 |
| DOIs | |
| Publication status | Published - 20 Feb 2004 |
| Event | 26th International Symposium on Capillary Chrmatography and Electrophoresis - LAS VEGAS, NEVADA Duration: 1 Jan 2004 → … |