Monitoring Allogeneic CAR-T Cells Using Flow Cytometry

Agnieszka Jozwik; Alan Dunlop; Katy Sanchez; Reuben Benjamin

doi:10.1007/978-1-0716-0203-4_19

Monitoring Allogeneic CAR-T Cells Using Flow Cytometry

Agnieszka Jozwik, Alan Dunlop, Katy Sanchez, Reuben Benjamin

KCH King's College Hospital NHS Foundation Trust

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Chimeric antigen receptor T cell (CAR-T) therapies have now entered mainstream clinical practice with two approved autologous CAR-T products targeting CD19 and numerous other products in early and late phase clinical trials. This has led to a demand for highly sensitive, specific, and easily reproducible methods to monitor CAR-T cells in patients. Here we describe a flow cytometry based protocol for detection of allogeneic CAR-T cells and for monitoring their phenotype and numbers in blood and bone marrow of patients following CAR-T treatment.

Original language	English
Pages (from-to)	293-308
Number of pages	16
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2097
DOIs	https://doi.org/10.1007/978-1-0716-0203-4_19
Publication status	Published - 1 Jan 2020

Keywords

Allogeneic
Cancer
CAR-T cells
Flow cytometry
Gene knockout
Immunotherapy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-0203-4_19

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AU - Benjamin, Reuben

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KW - Gene knockout

KW - Immunotherapy

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Monitoring Allogeneic CAR-T Cells Using Flow Cytometry

Abstract

Keywords

UN SDGs

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