TY - JOUR
T1 - Mouse monoclonal IgA binds to the galectin-3/Mac-2 lectin from mouse macrophage cell lines
AU - Reljic, R
AU - Crawford, C
AU - Challacombe, S
AU - Ivanyi, J
PY - 2004/4/30
Y1 - 2004/4/30
N2 - The beta-galactoside-binding S-type lectin galectin-3/Mac-2, expressed among several other cell types on activated macrophages, is known to bind IgE, but not other Ig classes. We report in this paper that the single major constituent in a detergent lysate from the J774 mouse macrophage cell line bound to a mouse monoclonal IgA-affinity column. This fraction has been identified by mass spectrometry analysis as galectin-3. Binding of both mouse IgA and IgE to galectin-3 coated plates was inhibited by lactose and asialofetuin. Furthermore, three different monoclonal IgAs bound also to purified recombinant hamster galectin-3 coated plates in a concentration dependent manner. The potential functional significance of IgA binding to galectin-3 within macrophages and possibly other cell types is discussed. (C) 2004 Elsevier B.V. All rights reserved.
AB - The beta-galactoside-binding S-type lectin galectin-3/Mac-2, expressed among several other cell types on activated macrophages, is known to bind IgE, but not other Ig classes. We report in this paper that the single major constituent in a detergent lysate from the J774 mouse macrophage cell line bound to a mouse monoclonal IgA-affinity column. This fraction has been identified by mass spectrometry analysis as galectin-3. Binding of both mouse IgA and IgE to galectin-3 coated plates was inhibited by lactose and asialofetuin. Furthermore, three different monoclonal IgAs bound also to purified recombinant hamster galectin-3 coated plates in a concentration dependent manner. The potential functional significance of IgA binding to galectin-3 within macrophages and possibly other cell types is discussed. (C) 2004 Elsevier B.V. All rights reserved.
UR - http://www.scopus.com/inward/record.url?scp=1842866684&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2004.01.015
DO - 10.1016/j.imlet.2004.01.015
M3 - Article
VL - 93
SP - 51
EP - 56
JO - Immunology Letters
JF - Immunology Letters
IS - 1
ER -