Mucosal immunization in macaques upregulates the innate APOBEC 3G anti-viral factor in CD4(+) memory T cells

Yufei Wang; Lesley A Bergmeier; Richard Stebbings; Thomas Seidl; Trevor Whittall; Mahavir Singh; Neil Berry; Neil Almond; Thomas Lehner

doi:10.1016/j.vaccine.2008.11.084

Mucosal immunization in macaques upregulates the innate APOBEC 3G anti-viral factor in CD4(+) memory T cells

Yufei Wang, Lesley A Bergmeier, Richard Stebbings, Thomas Seidl, Trevor Whittall, Mahavir Singh, Neil Berry, Neil Almond, Thomas Lehner

Research output: Contribution to journal › Article › peer-review

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Abstract

APOBEC3G is an innate intracellular anti-viral factor which deaminates retroviral cytidine to uridine. In vivo studies of APOBEC3G (A3G) were carried out in rhesus macaques, following mucosal immunization with SIV antigens and CCR5 peptides, linked to the 70 kDa heat shock protein. A progressive increase in A3G mRNA was elicited in PBMC after each immunization (p < 0.0002 to p ≤ 0.02), which was maintained for at least 17 weeks. Analysis of memory T cells showed a significant increase in A3G mRNA and protein in CD4+CCR5+ memory T cells in circulating (p = 0.0001), splenic (p = 0.0001), iliac lymph nodes (p = 0.002) and rectal (p = 0.01) cells of the immunized compared with unimmunized macaques. Mucosal challenge with SIVmac 251 showed a significant increase in A3G mRNA in the CD4+CCR5+ circulating cells (p < 0.01) and the draining iliac lymph node cells (p < 0.05) in the immunized uninfected macaques, consistent with a protective effect exerted by A3G. The results suggest that mucosal immunization in a non-human primate can induce features of a memory response to an innate anti-viral factor in CCR5+CD4+ memory and CD4+CD95+CCR7− effector memory T cells.

Original language	English
Pages (from-to)	870 - 881
Number of pages	12
Journal	Vaccine
Volume	27
Issue number	6
DOIs	https://doi.org/10.1016/j.vaccine.2008.11.084
Publication status	Published - 5 Feb 2009

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10.1016/j.vaccine.2008.11.084

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@article{21988b5d918b40dea4a63ea32149bdd0,

title = "Mucosal immunization in macaques upregulates the innate APOBEC 3G anti-viral factor in CD4(+) memory T cells",

abstract = "APOBEC3G is an innate intracellular anti-viral factor which deaminates retroviral cytidine to uridine. In vivo studies of APOBEC3G (A3G) were carried out in rhesus macaques, following mucosal immunization with SIV antigens and CCR5 peptides, linked to the 70 kDa heat shock protein. A progressive increase in A3G mRNA was elicited in PBMC after each immunization (p < 0.0002 to p ≤ 0.02), which was maintained for at least 17 weeks. Analysis of memory T cells showed a significant increase in A3G mRNA and protein in CD4+CCR5+ memory T cells in circulating (p = 0.0001), splenic (p = 0.0001), iliac lymph nodes (p = 0.002) and rectal (p = 0.01) cells of the immunized compared with unimmunized macaques. Mucosal challenge with SIVmac 251 showed a significant increase in A3G mRNA in the CD4+CCR5+ circulating cells (p < 0.01) and the draining iliac lymph node cells (p < 0.05) in the immunized uninfected macaques, consistent with a protective effect exerted by A3G. The results suggest that mucosal immunization in a non-human primate can induce features of a memory response to an innate anti-viral factor in CCR5+CD4+ memory and CD4+CD95+CCR7− effector memory T cells.",

author = "Yufei Wang and Bergmeier, {Lesley A} and Richard Stebbings and Thomas Seidl and Trevor Whittall and Mahavir Singh and Neil Berry and Neil Almond and Thomas Lehner",

year = "2009",

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T1 - Mucosal immunization in macaques upregulates the innate APOBEC 3G anti-viral factor in CD4(+) memory T cells

AU - Wang, Yufei

AU - Bergmeier, Lesley A

AU - Stebbings, Richard

AU - Seidl, Thomas

AU - Whittall, Trevor

AU - Singh, Mahavir

AU - Berry, Neil

AU - Almond, Neil

AU - Lehner, Thomas

PY - 2009/2/5

Y1 - 2009/2/5

N2 - APOBEC3G is an innate intracellular anti-viral factor which deaminates retroviral cytidine to uridine. In vivo studies of APOBEC3G (A3G) were carried out in rhesus macaques, following mucosal immunization with SIV antigens and CCR5 peptides, linked to the 70 kDa heat shock protein. A progressive increase in A3G mRNA was elicited in PBMC after each immunization (p < 0.0002 to p ≤ 0.02), which was maintained for at least 17 weeks. Analysis of memory T cells showed a significant increase in A3G mRNA and protein in CD4+CCR5+ memory T cells in circulating (p = 0.0001), splenic (p = 0.0001), iliac lymph nodes (p = 0.002) and rectal (p = 0.01) cells of the immunized compared with unimmunized macaques. Mucosal challenge with SIVmac 251 showed a significant increase in A3G mRNA in the CD4+CCR5+ circulating cells (p < 0.01) and the draining iliac lymph node cells (p < 0.05) in the immunized uninfected macaques, consistent with a protective effect exerted by A3G. The results suggest that mucosal immunization in a non-human primate can induce features of a memory response to an innate anti-viral factor in CCR5+CD4+ memory and CD4+CD95+CCR7− effector memory T cells.

AB - APOBEC3G is an innate intracellular anti-viral factor which deaminates retroviral cytidine to uridine. In vivo studies of APOBEC3G (A3G) were carried out in rhesus macaques, following mucosal immunization with SIV antigens and CCR5 peptides, linked to the 70 kDa heat shock protein. A progressive increase in A3G mRNA was elicited in PBMC after each immunization (p < 0.0002 to p ≤ 0.02), which was maintained for at least 17 weeks. Analysis of memory T cells showed a significant increase in A3G mRNA and protein in CD4+CCR5+ memory T cells in circulating (p = 0.0001), splenic (p = 0.0001), iliac lymph nodes (p = 0.002) and rectal (p = 0.01) cells of the immunized compared with unimmunized macaques. Mucosal challenge with SIVmac 251 showed a significant increase in A3G mRNA in the CD4+CCR5+ circulating cells (p < 0.01) and the draining iliac lymph node cells (p < 0.05) in the immunized uninfected macaques, consistent with a protective effect exerted by A3G. The results suggest that mucosal immunization in a non-human primate can induce features of a memory response to an innate anti-viral factor in CCR5+CD4+ memory and CD4+CD95+CCR7− effector memory T cells.

U2 - 10.1016/j.vaccine.2008.11.084

DO - 10.1016/j.vaccine.2008.11.084

M3 - Article

C2 - 19084567

SN - 1873-2518

VL - 27

SP - 870

EP - 881

JO - Vaccine

JF - Vaccine

IS - 6

ER -

Mucosal immunization in macaques upregulates the innate APOBEC 3G anti-viral factor in CD4(+) memory T cells

Abstract

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