Nanoparticle-based endodontic antimicrobial photodynamic therapy

Tom C Pagonis; Judy Chen; Carla Raquel Fontana; Harikrishna Devalapally; Karriann Ruggiero; Xiaoqing Song; Federico Foschi; Joshua Dunham; Ziedonis Skobe; Hajime Yamazaki; Ralph Kent; Anne C R Tanner; Mansoor M Amiji; Nikolaos S Soukos

doi:10.1016/j.joen.2009.10.011

Nanoparticle-based endodontic antimicrobial photodynamic therapy

Tom C Pagonis, Judy Chen, Carla Raquel Fontana, Harikrishna Devalapally, Karriann Ruggiero, Xiaoqing Song, Federico Foschi, Joshua Dunham, Ziedonis Skobe, Hajime Yamazaki, Ralph Kent, Anne C R Tanner, Mansoor M Amiji, Nikolaos S Soukos

Research output: Contribution to journal › Article › peer-review

204 Citations (Scopus)

Abstract

Objective
To study the in vitro effects of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the photosensitizer methylene blue (MB) and light against Enterococcus faecalis (ATCC 29212).

Materials and Methods
The uptake and distribution of nanoparticles in E. faecalis in suspension was investigated by transmission electron microscopy (TEM) after incubation with PLGA complexed with colloidal gold particles for 2.5, 5, and 10 minutes. E. faecalis species were sensitized in planktonic phase and in experimentally infected root canals of human extracted teeth with MB-loaded nanoparticles for 10 minutes followed by exposure to red light at 665 nm.

Results
The nanoparticles were found to be concentrated mainly on the cell walls of microorganisms at all three time points. The synergism of light and MB-loaded nanoparticles led to approximately 2 and 1 log10 reduction of colony-forming units (CFUs) in planktonic phase and root canals, respectively. In both cases, mean log10 CFU levels were significantly lower than controls and MB-loaded nanoparticles without light.

Conclusion
The utilization of PLGA nanoparticles encapsulated with photoactive drugs may be a promising adjunct in antimicrobial endodontic treatment.

Original language	English
Pages (from-to)	322-328
Number of pages	7
Journal	JOURNAL OF ENDODONTICS
Volume	36
Issue number	2
DOIs	https://doi.org/10.1016/j.joen.2009.10.011
Publication status	Published - Feb 2010

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10.1016/j.joen.2009.10.011

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@article{46e6f23cd7d24fbf935a6ecb470e2b16,

title = "Nanoparticle-based endodontic antimicrobial photodynamic therapy",

abstract = "ObjectiveTo study the in vitro effects of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the photosensitizer methylene blue (MB) and light against Enterococcus faecalis (ATCC 29212).Materials and MethodsThe uptake and distribution of nanoparticles in E. faecalis in suspension was investigated by transmission electron microscopy (TEM) after incubation with PLGA complexed with colloidal gold particles for 2.5, 5, and 10 minutes. E. faecalis species were sensitized in planktonic phase and in experimentally infected root canals of human extracted teeth with MB-loaded nanoparticles for 10 minutes followed by exposure to red light at 665 nm.ResultsThe nanoparticles were found to be concentrated mainly on the cell walls of microorganisms at all three time points. The synergism of light and MB-loaded nanoparticles led to approximately 2 and 1 log10 reduction of colony-forming units (CFUs) in planktonic phase and root canals, respectively. In both cases, mean log10 CFU levels were significantly lower than controls and MB-loaded nanoparticles without light.ConclusionThe utilization of PLGA nanoparticles encapsulated with photoactive drugs may be a promising adjunct in antimicrobial endodontic treatment.",

author = "Pagonis, {Tom C} and Judy Chen and Fontana, {Carla Raquel} and Harikrishna Devalapally and Karriann Ruggiero and Xiaoqing Song and Federico Foschi and Joshua Dunham and Ziedonis Skobe and Hajime Yamazaki and Ralph Kent and Tanner, {Anne C R} and Amiji, {Mansoor M} and Soukos, {Nikolaos S}",

year = "2010",

month = feb,

doi = "10.1016/j.joen.2009.10.011",

language = "English",

volume = "36",

pages = "322--328",

journal = "JOURNAL OF ENDODONTICS",

issn = "0099-2399",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Nanoparticle-based endodontic antimicrobial photodynamic therapy

AU - Pagonis, Tom C

AU - Chen, Judy

AU - Fontana, Carla Raquel

AU - Devalapally, Harikrishna

AU - Ruggiero, Karriann

AU - Song, Xiaoqing

AU - Foschi, Federico

AU - Dunham, Joshua

AU - Skobe, Ziedonis

AU - Yamazaki, Hajime

AU - Kent, Ralph

AU - Tanner, Anne C R

AU - Amiji, Mansoor M

AU - Soukos, Nikolaos S

PY - 2010/2

Y1 - 2010/2

N2 - ObjectiveTo study the in vitro effects of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the photosensitizer methylene blue (MB) and light against Enterococcus faecalis (ATCC 29212).Materials and MethodsThe uptake and distribution of nanoparticles in E. faecalis in suspension was investigated by transmission electron microscopy (TEM) after incubation with PLGA complexed with colloidal gold particles for 2.5, 5, and 10 minutes. E. faecalis species were sensitized in planktonic phase and in experimentally infected root canals of human extracted teeth with MB-loaded nanoparticles for 10 minutes followed by exposure to red light at 665 nm.ResultsThe nanoparticles were found to be concentrated mainly on the cell walls of microorganisms at all three time points. The synergism of light and MB-loaded nanoparticles led to approximately 2 and 1 log10 reduction of colony-forming units (CFUs) in planktonic phase and root canals, respectively. In both cases, mean log10 CFU levels were significantly lower than controls and MB-loaded nanoparticles without light.ConclusionThe utilization of PLGA nanoparticles encapsulated with photoactive drugs may be a promising adjunct in antimicrobial endodontic treatment.

AB - ObjectiveTo study the in vitro effects of poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the photosensitizer methylene blue (MB) and light against Enterococcus faecalis (ATCC 29212).Materials and MethodsThe uptake and distribution of nanoparticles in E. faecalis in suspension was investigated by transmission electron microscopy (TEM) after incubation with PLGA complexed with colloidal gold particles for 2.5, 5, and 10 minutes. E. faecalis species were sensitized in planktonic phase and in experimentally infected root canals of human extracted teeth with MB-loaded nanoparticles for 10 minutes followed by exposure to red light at 665 nm.ResultsThe nanoparticles were found to be concentrated mainly on the cell walls of microorganisms at all three time points. The synergism of light and MB-loaded nanoparticles led to approximately 2 and 1 log10 reduction of colony-forming units (CFUs) in planktonic phase and root canals, respectively. In both cases, mean log10 CFU levels were significantly lower than controls and MB-loaded nanoparticles without light.ConclusionThe utilization of PLGA nanoparticles encapsulated with photoactive drugs may be a promising adjunct in antimicrobial endodontic treatment.

U2 - 10.1016/j.joen.2009.10.011

DO - 10.1016/j.joen.2009.10.011

M3 - Article

C2 - 20113801

SN - 0099-2399

VL - 36

SP - 322

EP - 328

JO - JOURNAL OF ENDODONTICS

JF - JOURNAL OF ENDODONTICS

IS - 2

ER -

Nanoparticle-based endodontic antimicrobial photodynamic therapy

Abstract

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